最新刊期
卷 50 ， 期 5 ， 2025
- “空军军医大学西京医院联合多家机构，组织专家制定飞行人员血管源性脑白质高信号航空医学鉴定共识，旨在提供规范化和精准性文献支持及专家意见。”
摘要：White matter hyperintensities (WMH) are abnormal high-signal manifestations in white matter areas on magnetic resonance T2-weighted or fluid-attenuated inversion recovery (FLAIR) sequences, with vasogenic WMH being the most common. Its pathological mechanism is related to cerebrovascular lesions and can lead to multi-dimensional brain functional impairments in cognition, movement, and emotions. Due to their unique working conditions, aircrews are more prone to vasogenic WMH than the general population. Moreover, with the widespread use of magnetic resonance imaging in cranial disease screening for aircrews, the detection rate of vasogenic WMH in aircrews has significantly increased. However, to date, no national or academic organization has issued standardized aviation medical assessment criteria for vasogenic WMH in aircrews. Therefore, led by Xijing Hospital of Air Force Medical University and the Chinese PLA General Hospital, a multi-disciplinary expert panel involved in aviation medicine, neurology, radiology, psychology, and related fields was assembled to formulate this consensus. The document integrates evidence from a systematic review of PubMed, CNKI, Wanfang Data, and VIP databases, incorporates existing WMH guidelines, and synthesizes clinical expertise from Chinese specialists. The consensus outlines three key domains: target population, neuroimaging protocols for vasogenic WMH screening, and clinical assessment and aeromedical identification for aircrews with vasogenic WMH. The aim is to provide evidence-based support and expert recommendations for standardizing and improving the accuracy of aviation medical evaluations of vasogenic WMH in aircrews.关键词：aircrew;white matter hyperintensities;magnetic resonance imaging;medical assessment84|57|0更新时间：2026-01-28
Guideline and Consensus
- “据最新研究，中国恶性肿瘤负担仍重，发病率和死亡率均高于全球平均水平，需加强防治。”
摘要：ObjectiveTo compare the disease burden and evolving trends of major malignant tumors in China and globally from 1990 to 2021, and conduct predictive analysis, so as to provide a reference for formulating prevention and treatment policies for malignant tumor in China.MethodsBased on the Global Burden of Disease Database 2021 (GBD 2021), descriptive research methods were used to analyze the incidence and mortality of major malignant tumors in China and globally, as well as the changes in their rankings. The Joinpoint log-linear model was applied to analyze trends in age-standardized incidence rates (ASIR) and age-standardized mortality rates (ASMR) of malignant tumors in China and globally from 1990-2021. A Bayesian age-period-cohort (BAPC) model was constructed using R 4.4.0 to predict the incidence trends of malignant tumor in China and globally from 2022 to 2035.ResultsIn 2021, there were 17.2294 million new cases of malignant tumors (excluding non-melanoma skin cancer) and 9.7763 million deaths globally, with an ASIR of 201.06/100,000 and ASMR of 115.11/100,000. In China, 4.5375 million new cases and 2.7964 million deaths were reported, with ASIR and ASMR of 221.30/100,000 and 136.36/100,000, respectively. Lung cancer ranked first in both incidence and mortality of malignant tumors in China and globally. Female breast cancer had the second-highest ASIR in China. The number of incident cases and deaths from digestive system malignancies included in this study accounted for 42.0% of the total incident cases and 46.8% of the total deaths from all cancers in China, respectively. From 1990 to 2021, the average annual percentage change (AAPC) for overall ASIR of malignant tumors in China was 0.30, while ASMR declined (AAPC: -0.97). Globally, ASIR decreased slightly (AAPC: -0.10), and ASMR declined (AAPC: -0.78). The BAPC model predicted that by 2035, China's ASIR of malignant tumors may rise to 252.44/100,000, whereas the global ASIR is expected to decrease to 183.05/100,000.ConclusionsChina has achieved some success in cancer prevention and treatment, but still faces a heavy disease burden, with ASIR and ASMR exceeding global averages and ASIR persistently increasing. Cancer prevention and control work such as health education should be strengthened. Precise prevention and control measures should be taken for key cancer types, and preventive interventions should be focused on the elderly population.关键词：malignant tumor;incidence;morality;change trend;predictive analysis69|2028|0更新时间：2026-01-28 - “美国成年人群内脏肥胖指数与夜尿症关系研究显示，VAI与夜尿症发生风险正相关，为预测夜尿症风险提供有效指标。”
摘要：ObjectiveTo analyze the relationship between the visceral adiposity index (VAI) and nocturia in the US adult population.MethodsA cross-sectional study was performed. Data from subjects aged ≥20 years in the National Health and Nutrition Examination Survey (NHANES) database from 2007 to 2020 were collected, including waist circumference, triglyceride, body mass index (BMI), high-density lipoprotein, age, gender, race, poverty income ratio, education level, marital status, smoking, alcohol consumption, sleep disorders, depression, occupation, hypertension, diabetes, congestive heart failure, cancer, and nocturnal urination frequency. Weighted analysis, multivariate logistic regression, generalized additive model (GAM), and curve fitting were employed to evaluate the association between VAI and nocturia, adjusting for age, gender, race, poverty income ratio, education level, marital status, smoking, alcohol consumption, sleep disorders, depression, occupation, hypertension, diabetes, congestive heart failure, and cancer. Subgroup analyses were conducted based on age, gender, race, hypertension and diabetes to further evaluate the relationship between VAI and the risk of nocturia.ResultsA total of 29,196 American adults were included. All subjects were divided into 4 groups based on VAI quartiles: Q1 group (0.32≤VAI<1.01), Q2 group (1.01≤VAI<1.70), Q3 group (1.70≤VAI<2.95), and Q4 group (2.95≤VAI<13.59), with nocturia prevalence rates of 28.5%, 31.4%, 33.3%, and 34.9%, respectively. In subgroup analyses, the risk of nocturia significantly increased with higher VAI in the 20-40 age group, females and other Hispanics (OR=1.04, 95%CI 1.01-1.08, P=0.006; OR=1.02, 95% CI 1.00-1.04, P=0.035; OR=1.05, 95%CI 1.01-1.09, P=0.026). GAM analysis results showed a nonlinear relationship between VAI and nocturia.ConclusionVAI is positively associated with the risk of nocturia, and may be an effective indicator for predicting the risk of nocturia occurrence.关键词：nocturia;visceral obesity index;association;cross-sectional analysis64|1299|0更新时间：2026-01-28 - “在军事训练领域，研究者分析了身体运动功能评估系统在新兵训练伤风险评估中的应用，发现EMPF总分低于22分的新兵更易受伤。”
摘要：ObjectiveTo assess the effectiveness of the evaluation of military physical function (EMPF) system in predicting the occurrence of military training injuries among new recruits to provide scientific guidance and methodological choice for military training.MethodsA total of 527 new recruits from 5 grassroots units from July 2016 to February 2018 were selected for the study. The recruits underwent EMPF testing, and their military training injuries were monitored over a 2-year follow-up period. Those who sustained injuries during training were divided into injury group (n=163), while the remaining recruits were placed in healthy group (n=364). The predictive ability of the total EMPF score for training injuries was assessed using the receiver operating characteristic curve (ROC), and the correlation between the total EMPF score, individual test scores, and military training injuries were analyzed using binary logistic regression.ResultsThe total EMPF score of new recruits in injury group (19.52±1.97) was significantly lower than that of healthy group (24.31±1.54) (P<0.001), which also demonstrated a high diagnostic value in predicting the risk of military training injuries, with an area under the curve (AUC) of ROC of 0.971 (P<0.001). A cut-off value of 22 scores was found to have the highest accuracy in predicting future training injuries, with an odds ratio (OR) of 25.63, sensitivity of 0.939, specificity of 0.879, positive likelihood ratio of 7.76, and a post-test probability of 0.67. Binary logistic regression analysis revealed that 6 EMPF tests, including holding the ball over and leaning back, bending forward and touching the ground with the ball, lunge squat and twist, swallow balance with holding the ball afterward, vertical jump, and respiratory pattern assessment, were negatively associated with the risk of military training injuries (P<0.0001).ConclusionThe EMPF system can effectively predict the risk of military training injuries, with military personnel whose total EMPF score is less than 22 being at higher risk of sustaining such injuries.关键词：new recruits;physical function;evaluation;military training injury69|181|0更新时间：2026-01-28 - “南方医科大学南方医院重症医学科研究显示，脓毒症患者入科6小时的TRTG≤5.35℃是28天内死亡的危险因素，但体温梯度的改善与患者液体入量不相关。”
摘要：ObjectiveTo investigate the predictive value of temperature gradients on the mortality of sepsis patients and their correlation with fluid input.MethodsBy means of a prospective observational method, 154 patients with sepsis or septic shock admitted to the Department of Critical Care Medicine at Nanfang Hospital, Southern Medical University from November 2019 to November 2021 were included as research subjects. They were divided into a survivor group (n=118) and a non-survivor group (n=36) according to whether they survived within 28 days. The core-to-toe temperature gradient (CTTG) and toe-to-room temperature gradient (TRTG) were monitored and calculated immediately upon admission to the intensive care unit (ICU) and 6 hours after admission. Receiver operating characteristic (ROC) curve was used to explore the predictive value of temperature gradients on mortality, and multivariate Cox regression analysis was performed to explore the risk factors of 28-day mortality in sepsis patients. The results were verified through survival analysis. Correlation analysis and multivariate analysis of variance were used to explore the correlation between temperature gradients and fluid input, as well as noradrenaline doses.ResultsAmong the 154 patients, 118 survived within 28 days (survivor group), and 36 died (non-survivor group). ROC curve and multivariate Cox regression analysis showed that a toe-to-room temperature gradient of ≤5.35 ℃ within 6 hours after admission was a risk factor for 28-day mortality. Compared with patients with a high toe-to-room temperature gradient (>5.35 ℃), patients with a low toe-to-room temperature gradient (≤5.35 ℃) had a 2.74-fold increase in the risk of 28-day mortality (P=0.004, 95%CI 1.54, 9.12). The CTTG and TRTG upon admission to the ICU and 6 hours after admission were not significantly associated with fluid input or noradrenaline doses (P>0.05).ConclusionsA toe-to-room temperature gradient of less than or equal to 5.35 ℃ within 6 hours after ICU admission is a risk factor for 28-day mortality in sepsis patients. The improvement of temperature gradients at different time points is not associated with fluid input.关键词：sepsis;temperature gradient;fluid resuscitation;skin temperature49|213|0更新时间：2026-01-28 -
Systematic review of biologic therapy safety in pregnant and breastfeeding psoriasis patients 增强出版 AI导读
“在银屑病治疗领域，研究人员通过系统回顾和定量分析，评估了生物制剂在妊娠期及哺乳期的安全性。研究发现，妊娠及哺乳期女性银屑病患者使用生物制剂导致的不良妊娠结局未见明显增加，对新生儿健康及生长发育未见明显影响。”
摘要：ObjectiveTo systematically review and quantitatively analyze the safety of biologic agents for the clinical treatment of psoriasis during pregnancy and lactation.MethodsThe literature from start of database to June 27, 2023, was searched in MEDLINE (PubMed), Embase, Cochrane Library, and Web of Science by two researcher. Quality of included studies was assessed by the quality evaluation tool of case series from Australian JBI Evidence Based Healthcare Centre. According to Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA), a systematic review and was conducted to assess pregnancy or breastfeeding outcomes in psoriasis patients exposed to biologics within 3 months prior to pregnancy, during pregnancy or breastfeeding. Data on pregnancy and exposure characteristics were pooled, and the prevalence of adverse pregnancy outcome was summarized using a random effects model.ResultsA total of 54 studies involving 1206 pregnancies in 1177 female patients with psoriasis exposed to biologic agents were included in the analysis. Systematic review results demonstrated that the majority of the exposures were limited to early pregnancy, with pooled spontaneous abortion rates, elective abortion rates, overall mortality, preterm birth rates, incidence of low birth weights, and congenital anomalies similar to those of general population (P>0.05). Furthermore, no serious adverse reactions were reported during lactation.ConclusionsThe use of biologic agents in pregnant and breastfeeding women with psoriasis does not significantly increase the risk of adverse pregnancy outcomes and does not affect neonatal health or growth. However, the limited available safety data underscores the necessity of further studies to establish the relationship between psoriasis, biologic agents, and pregnancy/lactation outcomes, thereby providing comprehensive guidance for clinical practice.关键词：psoriasis;pregnancy;lactation;biological agents;safety57|489|0更新时间：2026-01-28 -
The application value of musculoskeletal ultrasound in grading acute gastrocnemius muscle injuries AI导读
“据中部战区总医院研究，肌骨超声(MSUS)可有效评估腓肠肌急性损伤分级，不同资历医师诊断结果一致性高，可作为首选检查方法。”
摘要：ObjectiveTo explore the application value of musculoskeletal ultrasound (MSUS) in grading acute gastrocnemius muscle injuries.MethodsA retrospective analysis was conducted on ultrasound images of 291 patients who presented with sudden calf pain and suspected acute gastrocnemius muscle injury in the General Hospital of Central Theater Command from March 2019 to July 2024. The images were independently reviewed and assessed by three ultrasound doctors with different qualifications to determine the presence and grade of gastrocnemius muscle injury. The consistency of grading results among three doctors was compared. The diagnostic results of three doctors were summarized. Then, the diagnostic results of 29 patients who underwent routine MRI scans were compared with those of MSUS, and the agreement between the two imaging modalities was assessed using the Kappa test.ResultsAmong the 291 patients, 171 cases (58.8%) were diagnosed with gastrocnemius muscle injury, including 55 cases (32.2%) with grade Ⅰ, 109 cases (63.7%) with grade Ⅱ, and 7 cases (4.1%) with grade Ⅲ. There were 159 cases (93.0%) of unilateral medial head injury, 10 cases (5.8%) of unilateral lateral head injury, and 2 cases (1.2%) of bilateral medial and lateral head injury. Compared with patients without gastrocnemius muscle injury, patients with gastrocnemius muscle injury were older (P<0.05), with no significant difference in gender and laterality (P>0.05). No significant differences in baseline characteristics were found among patients with different grades of injury (P>0.05). The three doctors diagnosed 173 (59.5%), 171 (58.8%), and 171 (58.8%) cases of injury, respectively, with an inter-class correlation coefficient (ICC) of 0.947 (P<0.001). Among 29 patients who underwent MRI, the diagnostic agreement between MRI and ultrasound for grade Ⅰ, Ⅱ and Ⅲ injury was 8 (27.6%), 18 (62.1%) and 3 (10.3%) for MRI; and 9 (31.0%), 17 (58.6%) and 3 (10.3%) for MSUS, respectively, with a Kappa value of 0.808 (P<0.001).ConclusionsMSUS is effective for assessing the grade of acute gastrocnemius muscle injury, and shows high diagnostic consistency among doctors with different qualifications. It is recommended as the preferred method for diagnosing gastrocnemius muscle injury.关键词：ultrasonography;musculoskeletal;gastrocnemius muscle;acute injury49|225|0更新时间：2026-01-28
Clinical Research
- “最新研究发现，降低宫颈癌细胞中Pin1表达能抑制肿瘤细胞干性及迁移侵袭能力，可能通过调控AP-1影响EMT发生。”
摘要：ObjectiveTo investigate the role of peptidyl prolyl cis-trans isomerase 1 (Pin1) in mediating stemness of tumor cells and the molecular mechanism of inducing epithelial-mesenchymal transition (EMT) in cervical cancer cells.MethodsThe Siha and Hele cells of Pin1 low-expression stable transfection uterine cervical neoplasm cell lines were constructed using lentivirus transfection technology and were divided into control group (shPin1-NON), knockdown group 1 (shPin1-1) and knockdown group 2 (shPin1-2). Western blotting and quantitative real-time polymerase chain reaction (qRT-PCR) were used to detect the expressions of Sex-determining region Y transcription factor 2 (SOX2), Aldehyde dehydrogenase 1A1 (ALDH1A1), and Cell adhesion molecule 44 (CD44). The serum-free spheroidization method was used to induce cervical cancer spheroids, with the adherent culture of cervical cancer cells as a control. Subsequently, Western blotting and qRT-PCR were employed to detect the expression of SOX2, ALDH1A1 and CD44 in both spheroid cells and adherent cells. Spheroid formation assay was used to detect the spheroid formation of cervical cancer cells after Pin1 knockdown. Transwell assay was used to detect the migration and invasion abilities of cervical cancer cells following down-regulation of Pin1. Western blotting and qRT-PCR were used to detect the expression of E-cadherin and N-cadherin attribute proteins in cervical cancer cells after transfection with pin1 low expression lentivirus. Western blotting and qRT-PCR were also used to assess the effects of Pin1 low expression on the expression levels of key proteins (c-Jun and c-Fos) of the transcriptional complex of Activator protein 1 (AP-1). Immunofluorescence combined with co-immunoprecipitation assays were conducted to detect the interaction and colocalization of Pin1 with c-Jun.ResultsIn Siha and Hele cells, the mRNA and protein expression levels of Pin1, SOX2, ALDH1A1 and CD44 in shPin1-NON group were significantly higher than those in shPin1-1 group and shPin1-2 group (P<0.05). The expression levels of SOX2, ALDH1A1 and CD44 mRNA and protein in cervical cancer spheroid group were significantly higher than those in adherent cervical cancer cells (P<0.05). Compared with shPin1-NON group, the spheroidism and migration invasion abilities of shPin1-1 group and shPin1-2 group were significantly reduced (P<0.05). Compared with shPin1-NON group, the mRNA and protein expressions of E-cadherin in shPin1-1 and shPin1-2 groups were significantly increased (P<0.05), while the mRNA and protein expression levels of N-cadherin were significantly decreased (P<0.05). The mRNA and protein expression levels of c-Jun and c-Fos in shPin1-NON group were significantly higher than those in shPin1-1 group and shPin1-2 group (P<0.05).ConclusionsDown-regulation of Pin1 can inhibit the stemness and migration invasion of cervical cancer cells, and Pin1 may mediate AP-1 to regulate the occurrence of stemness-induced epithelial-mesenchymal transition in cervical cancer cells.关键词：uterine cervical neoplasms;neoplastic stem cells;NIMA-interacting peptidylprolyl isomerase;epithelial-mesenchymal transition75|250|0更新时间：2026-01-28 - “最新研究发现，miR-185-5p通过靶向TM9SF1影响肺腺癌细胞增殖、迁移和自噬能力，为肺腺癌治疗提供新思路。”
摘要：ObjectiveTo investigate the effect of miR-185-5p-mediated targeted negative regulation of transmembrane 9 superfamily member 1 (TM9SF1) on proliferation, migration and autophagy in lung adenocarcinoma cells.MethodsThe expression of miR-185-5p in lung adenocarcinoma tissues was analyzed using dataset GSE51853 downloaded from the Gene Expression Omnibus (GEO) database. Potential target proteins of miR-185-5p were predicted using online databases (miRTargetLink, miRTarbase, and DIANA-microT-CD), and autophagy-related proteins were obtained from HADb. The intersected results from these four databases was identified, and survival curves of vascular endothelial growth factor A (VEGFA) and TM9SF1 within the overlapping candidates were analyzed using the StarBase database. TM9SF1 3'UTR wild-type (WT) or TM9SF1 3'UTR mutant (MUT) reporter plasmids were separately co-transfected with miR-185-5p control plasmid (CON) or miR-185-5p overexpression plasmid (over-miR-185-5p) into HEK-293T cells. A dual-luciferase reporter gene assay was employed to assess the binding interaction between miR-185-5p and TM9SF1 and quantify the subsequent luciferase activity. Western blotting was used to assess TM9SF1 protein expression levels in A549 cells transfected with over-miR-185-5p. A549 cells were divided into three groups: (1) CON+NC group, co-transfected with miR-185-5p control plasmid and TM9SF1 control plasmid; (2) over-miR-185-5p+NC group, co-transfected with over-miR-185-5p and TM9SF1 control plasmid; (3) over-miR-185-5p+over-TM9SF1 group, co-transfected with both miR-185-5p and TM9SF1 overexpression plasmids. EdU cell proliferation assay, wound healing assay, and Transwell migration assay were performed to validate the effects of miR-185-5p targeted binding to TM9SF1 on proliferation and migration capacities in lung adenocarcinoma. Changes in autophagic flux and mitochondrial membrane potential (MMP) of lung adenocarcinoma cells were detected using stubRFP-sensGFP-LC3 lentivirus and JC-1 assays, respectively.ResultsIn the GSE51853 dataset, miR-185-5p expression level was significantly lower in lung adenocarcinoma tissues compared with normal lung tissues (P<0.01). qRT-PCR analysis revealed that miR-185-5p expression was downregulated in lung adenocarcinoma cell lines NCI-H1299 and A549 compared with normal lung epithelial cells BEAS-2B (P<0.01). Bioinformatics predictions using miRTargetLink, miRTarbase, DIANA-microT-CD, and HADb databases indicated that miR-185-5p could target and regulate the autophagy-related protein TM9SF1. Dual-luciferase reporter assays and Western blotting demonstrated that miR-185-5p directly bound to the 3'UTR region of TM9SF1 mRNA, and overexpression of miR-185-5p significantly reduced the expression of target protein TM9SF1 (P<0.05). EdU cell proliferation, wound healing, and Transwell migration assays demonstrated that miR-185-5p overexpression inhibited proliferation and migration capacities of lung adenocarcinoma cells, whereas TM9SF1 overexpression could attenuate this inhibition effect (P<0.05). Results of stubRFP-sensGFP-LC3 for autophagic flux analysis demonstrated that overexpression of miR-185-5p enhanced autophagic flux in A549 cells, whereas co-overexpression of miR-185-5p and TM9SF1 suppressed autophagic flux. JC-1 assays showed a decreased MMP level in A549 cells after miR-185-5p overexpression, with higher MMP level observed when miR-185-5p and TM9SF1 were co-overexpressed.ConclusionmiR-185-5p may suppress proliferation, migration, and autophagy capacities in lung adenocarcinoma cells by targeting TM9SF1 through negative regulation.关键词：lung adenocarcinoma;miR-185-5p;transmembrane 9 superfamily protein 1;proliferation;migration;autophagy46|334|0更新时间：2026-01-28 - “最新研究发现，高龄妊娠子代出生后海马区突触修剪异常，表现为树突复杂性降低，树突棘密度降低、形态紊乱，可能是高龄妊娠子代脑功能障碍的机制之一。”
摘要：ObjectiveTo investigate the effects of advanced maternal age (AMA) on synaptic pruning of the hippocampus of offspring rats.MethodsOffspring of 3-month-old SD female rats and 3-month-old SD male rats were designated as the appropriate age group (Ctl group), while offspring of 12-month-old SD female rats and 3-month-old SD male rats were assigned to AMA group. Hippocampal tissues from both groups (n=3 per group at each time point) were subjected to Golgi staining on postnatal days (P) 7, 28, and 60. Dendritic complexity, spine density (DS), and morphology of immature brain neurons at different age stages were compared between the two groups.ResultsCompared with Ctl group, the dendritic length of AMA group on P7, P28, and P60 was significantly shortened, and the average number of intersections between dendrites and concentric circles on P28 and P60 was also significantly decreased (P<0.05). Compared with Ctl group, the dendritic spine density of AMA group was significantly decreased on P7, P28, and P60. Notably, the density of thin-shaped dendritic spines in AMA group was increased on P7, whereas the densities of stubby-shaped and mushroom-shaped dendritic spines were decreased (P<0.05). On P28 in AMA group, the densities of thin-shaped and mushroom-shaped dendritic spines were significantly increased compared with Ctl group, while the density of stubby-shaped dendritic spines was significantly decreased (P<0.05). The densities of stubby-shaped and mushroom-shaped dendritic spine were markedly lower in AMA group on P60 than those in Ctl group (P<0.05).ConclusionsDuring postnatal brain development periods, there were abnormalities in synaptic pruning in the hippocampal region of the offspring of the AMA, which manifested as reduced dendritic complexity, decreased dendritic spine density, and disorganized dendritic spine morphology. It could be one of the key mechanisms underlying brain dysfunction in the offspring of AMA.关键词：advanced maternal age;offspring rat;hippocampus;dendrites;neuronal plasticity54|121|0更新时间：2026-01-28 - “最新研究揭示香蒲新苷(TYP)通过调节SLC7A11-GPX4轴诱导肺腺癌细胞铁死亡，抑制其增殖、迁移和侵袭。”
摘要：ObjectiveTo investigate the effects and underlying molecular mechanisms of typhaneoside (TYP) on lung adenocarcinoma based on network pharmacology and in vitro experiments.MethodsTYP-lung adenocarcinoma-related genes were obtained from the prediction target website SwissTargetPrediction, The Cancer Genome Atlas (TCGA) database, GeneCards database, and Gene Expression Omnibus (GEO) database. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed on the obtained TYP-lung adenocarcinoma-related genes. Protein-protein network interactions analysis was carried out using the String database, and molecular docking was conducted with the Vina software. These network pharmacological analysis methods were employed to explore the theoretical action pathways of TYP on lung adenocarcinoma. PC9 cells were divided into control group (normal culture), low-TYP group (treated with 50 μmol/L TYP for 48 h), high-TYP group (treated with 100 μmol/L TYP for 48 h), and ferrostatin-1 (Fer-1)+TYP group (treated with 1 μmol/L Fer-1+100 μmol/L TYP for 48 h). The cell viability was detected by the CCK-8 assay, the cell proliferation ability was detected by the EdU assay, and the cell migration and invasion ability was detected by scratch assay, Transwell migration assay and Transwell invasion assay. The occurrence of ferroptosis was detected by reduced glutathione (GSH) assay kit, reactive oxygen species (ROS) assay kit, mitochondrial membrane potential assay kit, ferrous ion fluorescent probe, and transmission electron microscopy. Protein and mRNA expression levels of ferroptosis-related key molecules, solute carrier family 7 member 11 (SLC7A11) and glutathione peroxidase 4 (GPX4), were measured by Western blotting and reverse transcription-quantitative PCR (RT-qPCR). PC9 cells were transfected with SLC7A11 overexpression plasmid and divided into vector group (transfected with empty plasmid), vector+TYP group (transfected with empty plasmid and treated with 100 μmol/L TYP for 48 h), OE-SLC7A11 group (transfected with SLC7A11 overexpression plasmid) and OE-SLC7A11+TYP group (transfected with SLC7A11 overexpression plasmid and treated with 100 μmol/L TYP for 48 h). The molecular mechanism of SLC7A11 in the effect of TYP on PC9 was verified by CCK-8 assay, ferrous ion fluorescence probe, and protein expression levels of SLC7A11 and GPX4.ResultsA total of 73 TYP-lung adenocarcinoma-related target genes were predicted. GO analysis showed that the target genes were mainly involved in biological processes such as positive regulation of kinase activity, and were enriched in cellular components like transferase complex (transferring phosphorus-containing groups), revealing molecular functions such as transmembrane receptor protein tyrosine kinase activity. KEGG analysis identified 124 related pathways, mainly including epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor resistance pathway. Protein-protein interaction network analysis obtained 7 core targets such as serine/threonine kinase 1 (Akt1). Molecular docking results showed that the binding energies of TYP with core target genes and the ferroptosis-related proteins (SLC7A11 and GPX4) were all ≤-5.0 kcal/mol, indicating significant binding activity. CCK-8 assay showed that TYP significantly inhibited PC9 cell viability (P<0.05). EdU assay demonstrated that the proportion of proliferating cells in TYP group was significantly lower than that in control group (P<0.05). Scratch assay, Transwell migration assay, and invasion assay revealed that, compared with control group, the migration and invasion abilities of PC9 cells in TYP group significantly decreased (P<0.05), and GSH content and mitochondrial membrane potential in TYP group also significantly decreased (P<0.05), while ROS and Fe2+ contents increased significantly (P<0.01), and protein and mRNA expression levels of SLC7A11 and GPX4 decreased significantly (P<0.05). Transmission electron microscopy results showed that cells in TYP group exhibited specific ferroptosis-related changes such as reduced mitochondrial cristae and increased membrane density, compared with control group. Compared with vector+TYP group, OE-SLC7A11+TYP group had higher cell viability (P<0.05), lower Fe2+ content (P<0.05), and higher protein expression levels of SLC7A11 and GPX4 (P<0.05).ConclusionTYP may induce ferroptosis in lung adenocarcinoma cells and inhibit their malignant behaviors such as proliferation, migration and invasion by regulating the SLC7A11-GPX4 axis.关键词：ferroptosis;typhaneoside;lung adenocarcinoma;network pharmacology;molecular docking45|1087|0更新时间：2026-01-28 - “最新研究发现，100 mT静磁场连续暴露14天、每天0.5小时，可导致小鼠产生神经炎症及脑损伤，但未见焦虑样行为。”
摘要：ObjectiveTo investigate the effects of a 100 mT static magnetic field (SMF) on emotional behavior and brain damage-related molecules in mice.MethodsFifty-eight C57BL/6N mice were randomly divided into control group (n=25) and observation group (n=33). Mice in observation group were exposed to a 100 mT SMF for 0.5 h/d over 14 consecutive days, while mice in control group underwent pseudo-exposure. On the 7 and 14 days of exposure, anxiety-like behavior was assessed using open field and elevated plus maze tests. Cerebral blood flow was monitored using laser speckle imaging, and the levels of tumor necrosis factor-α(TNF-α), interleukin (IL)-1β, IL-4, central nervous system specific protein β (S100β), neuron-specific enolase (NSE), and brain-derived neurotrophic factor (BDNF) were measured by radioimmunoassay. BDNF expression in the brain was detected by immunofluorescence.ResultsOn the 7 and 14 days of SMF exposure, the open field and elevated plus maze tests showed no statistically significant differences between observation and control groups in the frequencies, durations, and distance entering the central area of the open field and the open arm of the elevated plus maze (P>0.05). Laser speckle imaging revealed no significant difference in cerebral cortical perfusion compared with pre-exposure period (P>0.05). The results of radioimmunoassay showed that compared with control group, on the 7 d of SMF exposure, the serum IL-1β, NSE and S100β levels were significantly increased (P<0.05), the serum BDNF level was significantly decreased (P<0.05), and the IL-1β and TNF-α contents in brain tissues were significantly increased in observation group (P<0.01). On the 14 d of SMF exposure, serum IL-1β, TNF‑α, NSE, and S100β levels were significantly increased (P<0.05, P<0.0001), and the brain IL-1β and TNF‑α levels were significantly increased (P<0.01) in observation group. No statistically significant differences were found in anti-inflammatory cytokine IL-4 level of serum and brain tissue or BDNF content of brain tissue between the two groups (P>0.05).ConclusionContinuous exposure to a 100 mT SMF for 14 d at 0.5 h/d induces neuroinflammation and brain damage in mice, without inducing anxiety-like behavior.关键词：static magnetic field;anxiety;blood flow;Brain injury;inflammation34|193|0更新时间：2026-01-28 - “最新研究发现，运动习服能降低劳力型热射病发病率，提高生存率，减轻肝损伤和炎症反应。”
摘要：ObjectiveTo compare the effects of different exercise acclimatization (EA) durations on liver injury and inflammatory response in mice with exertional heatstroke (EHS).MethodsA total of 168 male C57BL/6 mice were randomly assigned to four groups using a random number table: no exercise acclimation group (EA0W, n=54), 1-week exercise acclimation group (EA1W, n=54), 2-week exercise acclimation group (EA2W, n=54), and blank control group (n=6). The blank control group did not undergo acclimatization training or EHS modeling. The EA1W and EA2W groups underwent daily 2-hour exercise training at a speed of 10 m/min in an environment maintained at (26.0±0.5) ℃ for 1 and 2 weeks, respectively, followed by a 2-day rest after training completion. EHS modeling was performed in mice of EA0W, EA1W, and EA2W groups through running at 10 m/min under controlled environmental conditions (39.5 ℃ ambient temperature, 65% relative humidity). The modeling endpoint was defined as loss of consciousness accompanied by a core body temperature ≥42.7 ℃. All modeling procedures were systematically documented. Following modeling, 18 mice from EA0W, EA1W, and EA2W groups underwent 24-hour survival analysis. Blood samples from the abdominal aorta and liver tissues were collected at 6, 12 and 24 hours post-modeling (6 mice per time point for each group). Plasma levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and creatine kinase (CK) were quantified. Interleukin (IL)-1β and IL-6 concentrations were determined using enzyme-linked immunosorbent assay (ELISA). Liver tissue specimens underwent hematoxylin-eosin (HE) staining and pathological scoring.ResultsThe EHS model was successfully established in all EA groups. When all mice in EA0W group developed EHS (65 min after the modeling initiation), the incidence rates in EA1W and EA2W groups were 50.0% and 22.2%, respectively, with a statistically significant difference between EA0W group and the latter two groups (P<0.05). When all mice in the three groups developed EHS, the time to EHS onset was significantly longer in both EA1W and EA2W groups compared to EA0W group, with EA2W group showing a longer onset time than EA1W group (P<0.05). Survival analysis revealed a significantly higher 24-hour survival rate in EA2W group (61.1%) compared to EA0W group (33.3%) (P<0.05), while no significant difference was observed between EA1W group and the other two groups (P>0.05). The levels of IL-1β, IL-6, and CK were highest at 6 h post-modeling in all EA groups (P<0.05), and liver injury was most severe at 12 h post-modeling (P<0.05). Compared to EA0W group, the levels of ALT, AST, and IL-1β, as well as liver pathology scores, were significantly lower at 12 h post-modeling in both EA1W and EA2W groups (P<0.05), with EA2W group showing significantly lower ALT and AST levels, as well as liver pathology scores than EA1W group (P<0.05). At 6 h post-modeling, CK levels were significantly higher in EA1W and EA2W groups compared to EA0W group (P<0.05), with EA2W group exhibiting higher CK levels than in EA1W group (P<0.05).ConclusionsExercise acclimation helps reduce the incidence of EHS. Following EHS onset, the survival rate of exercise-acclimated mice is higher than that non-acclimated mice, with a significantly higher survival rate in mice acclimated for 2 weeks compared to non-acclimated mice. However, no significant difference in survival rate is observed between mice acclimated for 1 week and non-acclimated mice. Additionally, exercise acclimation for 2 weeks is more effective in reducing liver injury and inflammatory responses compared to 1-week acclimation.关键词：heat stroke;acclimatization;liver injuries;inflammation40|283|0更新时间：2026-01-28
Basic Research
- “转录组测序技术揭示子宫内膜容受性基因表达，助力辅助生殖技术发展。”摘要：Good endometrial receptivity is an essential factor for embryo implantation, and gene expression in endometrial tissue during the window of implantation (WOI) is closely related to receptivity. Transcriptome sequencing technology enables the identification of gene expression profiles of endometrium during different menstrual phases, as well as microRNAs and long-chain non-coding RNA sequences involved in regulating gene expression. Combining this technology with bioinformatics analysis provides a better understanding of specific gene expression during the receptive period and offers technical support for studying its regulatory mechanism. Moreover, gene expression profiles of the endometrium during different menstrual phases hold significant clinical application value for accurately assessing endometrium receptivity in infertility patients and those with repeated implantation failure, thereby guiding individualized embryo transfer strategies. This review summarizes the progress of transcriptome sequencing in evaluating human endometrial receptivity and discusses future research directions. This review aims to understand the complex molecular mechanisms of endometrial receptivity formation and regulation from the transcriptional level, in order to improve the implantation rate of embryos in assisted reproductive technology and reduce the abortion rate.关键词：endometrium;RNA sequencing;embryo transfer;endometrial receptivity63|415|0更新时间：2026-01-28
- “热射病研究新进展，专家探讨发病机制和降温治疗，为临床救治提供参考。”摘要：Heat stroke is a heat-related illness caused by an imbalance between the body's heat production and heat dissipation, which could lead to multiple organ dysfunction syndrome with a high mortality rate. Rapid and effective reduction of core body temperature is key to successful treatment. This article reviews recent progress in the treatment of heat stroke, including new understandings of organ injury mechanisms, the timing, velocity and goals of cooling treatment, evaluation and selection of traditional cooling techniques (such as cold water immersion), and scientific evaluation of new cooling technologies (such as blood purification technology and intravascular heat exchange cooling technology), aiming to promote understanding and treatment of heat stroke.关键词：heat stroke;organ injury;cooling;endovascular heat exchange cooling131|968|0更新时间：2026-01-28
- “最新研究揭示了肠道菌群与慢性肾脏病炎症状态的关联，并探讨了益生菌等干预策略的潜力，为治疗提供新思路。”
摘要：The inflammatory status in patients with chronic kidney disease (CKD) is closely associated with cardiovascular events, infections, and other complications, and is a powerful indicator for prognosis assessment. The core view of the "gut-kidney axis" theory reveals the relationship among inflammatory state, microbiota dysbiosis, and deterioration of renal function. The microbiota alters the microenvironment through structural changes and metabolites with different properties, subsequently leading to microbiota translocation, inducing inflammatory lesions, and damaging the kidneys. Recent studies have proposed that targeted microbiota intervention strategies such as probiotics, prebiotics, and synbiotics can modulate the microbiota structure, regulate the microenvironment, relieve renal inflammation, and affect the progression of renal disease, representing a potentially promising research direction in the future. This review discusses the characteristics of how intestinal microbiota influence the inflammatory status in CKD, focusing on the research progress of targeted microbiota intervention, aiming to discuss the effectiveness and scientific basis of these strategies, providing a foundation for the treatment of CKD and the expansion of targeted microbiota research, as well as offering references for the clinical application of probiotics, prebiotics, and synbiotics.关键词：chronic kidney disease;inflammation;probiotics;prebiotics;synbiotics57|542|0更新时间：2026-01-28 - “阿尔茨海默病研究取得新进展，为临床应用提供参考。”
摘要：Alzheimer's disease (AD), a major cause of dementia, is a common neurodegenerative disease, beginning with memory loss and difficulties with thinking, language and problem-solving skills. Intensification of population aging and the increasing incidence of AD have imposed a heavy burden on healthcare systems. Currently, the main pathological explanation remains the excessive accumulation of β‑amyloid plaques, formation of neurofibrillary tangles and neuronal loss, but the true etiology and pathogenesis of AD remain unknown. The advances of research in AD, with respect to genetics, pathophysiological mechanism, imaging diagnosis and laboratory diagnosis, are reviewed in this article, aiming to provide references for relevant research and potential clinical applications.关键词：Alzheimer's disease;cognitive impairment;imaging diagnosis;biomarkers74|2083|0更新时间：2026-01-28
Review
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