最新刊期
卷 50 ， 期 1 ， 2025
- “甘肃省人民医院研究显示，基于MRI的前列腺癌影像组学对骨转移诊断具有高价值，结合临床特征可提高诊断能力。”
摘要：ObjectiveTo investigate the diagnostic value of magnetic resonance imaging (MRI)-based intratumoral and peritumoral radiomics of prostate cancer (PCa) for bone metastases.MethodsA total of 211 patients diagnosed with PCa by biopsy pathology at Gansu Provincial People's Hospital from January 2018 to January 2023 were retrospectively analyzed. These patients were randomly divided into a training set (n=147) and a validation set (n=64) in a 7:3 ratio. Regions of interest (ROIs) were delineated from the patients' T2-weighted imaging (T2WI), diffusion-weighted imaging (DWI), and apparent diffusion coefficient imaging (ADC) sequences to extract radiomic features. Z-score (normalization) and the LASSO algorithm were used for feature dimensionality reduction, selection, and construction. A predictive model was then built using a logistic regression (LR) machine learning classifier. The receiver operating characteristic (ROC) curve was plotted, and the area under the curve (AUC) was calculated to assess the model's performance. Calibration curves and decision curves (DCA) were plotted to evaluate the model's fit and clinical net benefit.ResultsRadiomic features were extracted from the tumor and peritumoral regions in each patient's T2WI, DWI, and ADC images, with a total of 312 features from each region. The LASSO regression model ultimately identified 10 intratumoral radiomic features closely related to bone metastasis, including 2 T2 sequence features, 7 DWI features, and 1 ADC sequence feature; and 9 peritumoral radiomic features, including 4 T2 sequence features, 3 DWI features, and 2 ADC sequence features. The predictive model based on intratumoral radiomic features achieved an AUC of 0.845 (95%CI 0.747-0.943), while the predictive model based on peritumoral radiomic features had an AUC of 0.818 (95%CI 0.716-0.919). A combined nomogram model incorporating intratumoral features, peritumoral radiomic features, and clinical features (including Gleason score, total prostate specific antigen, and body mass index) yielded an AUC of 0.936 (95%CI 0.902-0.970). Calibration curves indicated that the combined model had good fit, and DCA demonstrated that the combined model provided better clinical net benefit.ConclusionsPeritumoral radiomics has excellent predictive value for bone metastasis in newly diagnosed PCa. Combining with intratumoral radiomics features and clinical features, it significantly enhances the predictive capability of the model.关键词：prostate cancer;bone metastases;peritumoral radiomics;machine learning9|0|0发布时间：2025-01-23 - “最新研究显示，人工智能在膝骨关节炎诊疗中具有巨大潜力，可提升诊断效率和预测准确性，但需优化算法和加强验证。”摘要：Knee osteoarthritis (KOA) is a chronic degenerative joint disease, which poses a major challenge particularly among the elderly population due to its high incidence and high disability. Imaging examination has been used commonly to diagnose KOA. However, it faces imitations in predicting disease progression due to the lack of prior information and constraints in manpower and time. With the rapid evolution of big data and computational technologies, artificial intelligence (AI) is progressively integrating into various healthcare domains. Therefore, the integration of artificial intelligence (AI) into healthcare holds promise for revolutionizing KOA diagnosis and treatment. AI-assisted diagnostic models have demonstrated the potential to automate diagnosis, classify disease severity, and predict disease progression with improved efficiency and accuracy. In addition, these models provide personalized diagnosis and treatment options, as well as accurate disease progression risk assessment. Despite these promising outcomes, challenges such as high costs associated with data annotation and limitations in model generalization capabilities persist. This paper reviews recent advancements in AI applications and summarizes the potential value of utilizing AI applications for KOA. To further enhance the utilization of AI in KOA management to overcome current limitations, future efforts should focus on standardizing clinical sample databases, optimizing AI algorithms, and enhancing external verification sets.关键词：osteoarthritis;artificial intelligence;machine learning;deep learning20|0|0发布时间：2025-01-23
- “最新研究显示，内镜光学相干断层扫描成像技术结合人工智能，在消化道肿瘤诊断领域具有巨大潜力，有望提高患者生存率和生活质量。”摘要：Diagnosis and treatment of digestive tract tumors is not optimistic with high incidence and mortality. Early diagnosis is conducive to improving the survival rate and quality of life of the patients. A variety of endoscopic imaging techniques have been applied in the diagnosis of digestive tract tumors, each with its own advantages and disadvantages. Optical coherence tomography (OCT) has emerged as a non-invasive and high-resolution imaging technique with unique advantages in staging and diagnosis of superficial digestive tract tumors. The complexity of massive image processing has also been effectively addressed with the development of artificial intelligence (AI), and AI-assisted OCT imaging, especially in the diagnosis of digestive tract tumors, has shown good prospects. This review summarizes the principle and development of OCT, and discusses the potential of AI-assisted OCT for deep learning in the diagnosis of digestive tract tumors.关键词：Optical coherence tomography;digestive tract tumors;artificial intelligence;diagnosis10|0|0发布时间：2025-01-23
- “在现代战场环境下，腹部战创伤发生率高、病情变化快、病死率高，急需新医疗技术提高急救效率和成功率。智能医疗的发展为腹部战创伤诊治提供新思路。”摘要：Abdominal war trauma is a common and high-risk type of injury in the modern battlefield, with rapid changes in condition and a high mortality rate. There is an urgent need for emerging medical technologies to improve the efficiency and success rate of first aid for military casualties. With the development of artificial intelligence (AI), 5G, and other emerging technologies, the concept of intelligent medical treatment is gradually forming and can assist in the diagnosis and treatment of abdominal trauma. This paper reviews the characteristics of abdominal war trauma in modern wars, discusses the application of intelligent medical treatment for abdominal war trauma and its drawbacks to be solved, aiming to provide reference for research related to abdominal war trauma.关键词：intelligent medicine;abdominal war trauma;artificial intelligence;5G;laparoscopic surgery15|0|0发布时间：2025-01-23
Special Issue on Application of Artificial Intelligence in Disease Diagnosis and Treatment
- “最新研究发现，胆囊结石与小肠细菌过度生长(SIBO)存在明显关联，年龄、BMI、血糖水平升高等是胆囊结石的独立危险因素。”摘要：ObjectiveTo explore the correlation between gallbladder stones and small intestinal bacterial overgrowth (SIBO).MethodsA retrospective analysis was conducted on the clinical data of 393 patients who attended the Department of Gastroenterology of the Sixth Medical Center of Chinese PLA General Hospital from January 2021 to September 2023. They were divided into gallbladder stones group (n=190) and control group (n=203) based on the presence of gallbladder stones. Their general clinical data, laboratory test results, and abdominal symptoms were compared. Multivariate logistic regression was used to analyze the risk factors for gallbladder stones. Additionally, the total population was divided into SIBO-positive group (n=239) and SIBO-negative group (n=154), and their clinical characteristics were analyzed by logistic regression to explore the risk factors for SIBO.ResultsUnivariate analysis revealed that gallbladder stones group had a higher rate of age, body mass index (BMI), fasting plasma glucose (FPG), glutaminase levels, prevalence of hypertension, diabetes, coronary heart disease, non-alcoholic fatty liver disease, gallbladder polyps, and SIBO, as well as a higher prevalence of CH4-positive and H2-positive in SIBO group than control group (P<0.05). In terms of abdominal symptoms, the incidence of bad breath (48.4% vs. 35.5%), dyspepsia (38.4% vs. 28.6%), abdominal pain (30.5% vs. 14.8%), bloating (42.1% vs. 28.6%), diarrhea (20.5% vs. 7.4%), and more exhaustion (46.8% vs. 34.5%) were significantly higher in gallbladder stones group than those in control group (P<0.05). Multivariate logistic regression analysis showed that independent positive determinants for incident gallbladder stones were age, BMI, FPG, total bilirubin (TBIL), coronary heart disease, gallbladder polyps, and SIBO. Univariate analysis revealed that age, prevalence of gallbladder stones, proportion of single stones, triglycerides (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) were significantly higher in SIBO-positive group than those in SIBO-negative group (P<0.05). Multivariate logistic regression analysis showed that the risk factors for SIBO were age, coronary heart disease, and gallbladder stones, while the protective factor for SIBO was high-density lipoprotein cholesterol (HDL-C).ConclusionThere is a significant correlation between gallbladder stones and small SIBO; interventions on related factors of gallbladder stones and small SIBO may help reduce their incidence.关键词：cholelithiasis;gallbladder stones;small intestinal bacterial overgrowth;breath test11|0|0发布时间：2025-01-23
- “最新研究发现，慢性鼻窦炎鼻息肉患者血浆CYBB、CSF1水平升高，可能成为诊断标志物。”
摘要：ObjectiveTo analyze the gene expression characteristics of chronic rhinosinusitis with nasal polyps (CRSwNP) using bioinformatics methods, aim to investigate the potential biomarkers and their diagnostic value of CRSwNP.Methods(1) The CRSwNP Gene expression data set was downloaded from the American Gene Expression Omnibus (GEO) database. The differentially expressed genes (DEGs) between CRSwNP patients and healthy controls were screened through data analysis. Gene Ontology (GO) functional enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were performed on the identified DEGs. Protein-protein interaction (PPI) networks were constructed utilizing the STRING database, and the key genes were identified by using the cytoHubba plugin. The "Cibersort" package was used to analyze the influence of key genes on common immune cells. (2) Thirty-two patients diagnosed with CRSwNP in the First Affiliated Hospital of Hebei North University from June 2022 to June 2023 were selected as the CRSwNP group, and 21 patients with simple deviation of nasal septum without a history of sinusitis during the same period were selected as control group. The pathological characteristics of specimens in the two groups were examined using hematoxylin-eosin (HE) staining. Immunohistochemistry and Western blotting were used to detect the expression levels of key genes in CRSwNP. The levels of key proteins in plasma were detected using ELISA, and ROC curve was used to analyze its efficacy in diagnosing CRSwNP.Results(1) Analysis of three gene expression database sets (GSE36830, GSE23552, and GSE194282) showed that there were 156 DEGs in CRSwNP. GO functional enrichment and KEGG pathway analysis indicated that the functions of the above DEGs were mostly related to immune functions. Key genes such as cytochrome b-245 β chain (CYBB) and colony-stimulating factor 1 receptor (CSF1R) were identified. (2) The results of HE staining revealed that the epithelial of CRSwNP tissue was metaplastic into stratified squamous epithelium with interstitial edema. Both immunohistochemistry and Western blotting analyses indicated that the expression levels of CYBB and CSF1R in the CRSwNP group were significantly increased compared to control group (P<0.05). ELISA results demonstrated that CYBB [(21.20±3.00) μg/ml vs. (17.66±1.66) μg/ml, P<0.05] and CSF1 [(477.37±86.63) pg/ml vs. (370.71±66.24) pg/ml, P<0.05] in CRSwNP group were significantly increased compare to control group. ROC curve analysis showed that plasma concentrations of CYBB and CSF1 had AUCs of 0.888 (95%CI 0.802-0.974) and 0.821 (95%CI 0.711-0.931) for diagnosing of CRSwNP, respectively; their combined AUC was 0.927 (95%CI 0.851-1.000).ConclusionsCYBB and CSF1R may be involved in the occurrence and development of CRSwNP. Plasma CYBB and CSF1 have high diagnostic value for CRSwNP.关键词：CRSwNP;hub genes;bioinformatics;screening and diagnosis11|0|0发布时间：2025-01-23 - “在前列腺癌治疗领域，河北北方学院附属第一医院放疗科专家通过前瞻性随机对照临床研究，验证了剂量表面积直方图(DSH)对急性放射性直肠炎的预测效能，与剂量体积直方图(DVH)相似，为放射治疗计划设计提供新方案。”
摘要：ObjectiveTo evaluate the predictive value of dose-surface histogram (DSH) for radiation proctitis (RP) in prostate cancer (PCa) patients undergoing radiotherapy.MethodsThis prospective randomized controlled clinical trial included 380 PCa patients who underwent image-guided radiotherapy in the First Affiliated Hospital of Hebei Northern University from January 2018 to January 2023. Patients were randomly divided into observation group (n=200) and control group (n=180). The rectal dose distribution of patients in the two groups was evaluated by using DSH and dose-volume histogram (DVH), respectively. The receiver operating characteristic (ROC) curve was utilized to evaluate the predictive value of DSH for acute RP, with DVH serving as a reference.ResultsThe difference was not statistically significant in clinical information such as age, KPS score, and body mass index (BMI) between the observation and control groups (P>0.05), as well as in acute RP incidence (P>0.05). There were significant differences in S40 and V40, S50 and V50, S60 and V60, S70 and V70, and S78 and V78 between the two groups (P<0.05). S40, S50, V40, and V50 showed low efficacy (P<0.001) in predicting acute RP at each level, with AUC ≤0.700. S60 and V60 showed moderate efficacy(P<0.001) in predicting acute RP at each level, with AUC 0.700-0.900. S70, S78, V70 and V78 showed high efficacy (P<0.001) in predicting acute RP at each level, with AUC >0.900.ConclusionsThe predictive value of DSH for rectal toxicity in patients with PCa is basically consistent with that of DVH. It is expected to become a novel and valuable tool for evaluating radiotherapy plans in the future.关键词：dose and surface histogram;prostate cancer;rectal toxicity;forecast18|0|0发布时间：2025-01-23 - “在性发育异常诊治领域，河北北方学院附属第一医院报道了一例染色体核型与基因检测结果不符的病例，通过多胚层细胞取样和荧光原位杂交技术，揭示了性腺中Y染色体的分布差异，为性发育异常嵌合状况的认识提供了新见解。”
摘要：ObjectiveTo report the diagnosis, treatment, and verification process of a patient with sex development disorder whose chromosomal karyotype and genetic test results are inconsistent, and conduct a literature review to improve the understanding of the mosaic status of sexual development disorders.MethodsA 14-year-old patient presented with primary amenorrhea on April 3, 2020, at the First Affiliated Hospital of Hebei North University, exhibiting female sexual characteristics. The patient underwent ultrasonic/magnetic resonance imaging of gonads, assessment of gonadal axis function, chromosomal karyotype, and molecular genetic testing, as well as pelvic exploration, malignant gonads resection, and hormone replacement therapy, resulting in drug-induced menstruation. During the diagnosis and treatment, it was found that the patient's chromosomal karyotype analysis was inconsistent with the molecular genetic test results. Subsequently, samples from the three germ layer cells were taken, and fluorescence in situ hybridization (FISH) was used to detect the sex chromosomes in each germ layer cell. XY probes were used to label the gonadal pathological sections to explore the distribution differences of the Y chromosome in the gonads, and changes in anti-Müllerian hormone (AMH) levels before and after surgery were compared. Databases such as Wanfang and PubMed were searched to summarize relevant cohort study literature and understand the current status of research on this disease.ResultsThe patient's body exhibited a significant differences between the 45,X and 46,XY cell lines in different germ layers and within the same layer tissues. The proportion of 45,X in buccal mucosal cells derived from the ectoderm was 30% (6/20), in peripheral blood lymphocytes derived from the mesoderm was 9.7% (11/114), and in bladder shed cells derived from endoderm was 20.4% (22/108). The gonadal pathological sections labeled with XY probes indicated a mosaic state with a reduced Y-chromosome; where the epididymal structure area had a 45,X cell line mosaic of 50.0%, and the malignant area had a normal "Y" content. After gonadal resection, AMH levels significantly dropped from 7.28 pmol/L to <0.07 pmol/L. Literature review revealed that patients with 45,X/46,XY have a complex phenotype spectrum, most with features of Turner syndrome, and female phenotypes are at risk of gonadal tumors.ConclusionsIn the diagnosis of difficult cases of sex development disorders, when performing peripheral blood karyotype testing, the number of counted cells and analyzed cells should be increased as much as possible, and multi-germ layer cell sampling should be performed. Gonads with a high "Y" mosaic rate are more prone to malignancy in the abdominal cavity. Detecting AMH levels can distinguish cryptorchidism and anorchidism in sexual development disorders with Y chromosomes.关键词：sex development disorder;chromosome mosaicism;gonadal tumor;anti-Müllerian hormone(AMH);mosaic10|0|0发布时间：2025-01-23
Clinical Research
- “最新研究发现，SIRT7高表达与胶质瘤患者预后差相关，能通过TGF-β/Smad3通路促进胶质瘤细胞增殖和迁移。”
摘要：ObjectiveTo investigate the expression of the histone deacetylase SIRT7 in glioma cells and its impact on epithelial-mesenchymal transformation (EMT), as well as its effects on proliferative, migratory and invasive capabilities of glioma cells.MethodsBioinformatics analysis was conducted on data from glioma patients in the Cancer Genome Atlas (TCGA) and the Chinese glioma Genome Atlas (CGGA) databases to explore the expression of SIRT7 gene in gliomas and its correlation with tumor grading, molecular characteristics and patient clinical prognosis. Glioma cells were randomly divided into control, SIRT7 knockdown, SIRT7 overexpression, drug treatment (10 μmol/L hydrochlorothiazide) and drug (10 μmol/L hydrochlorothiazide)+SIRT7 overexpression groups. The CCK-8 assay, cell scratch assay and Transwell assay were used to observe the effects of upregulating and downregulating SIRT7 expression on glioma cell proliferation, migration and invasion. RT-qPCR and Western blotting were employed to detect the effects of SIRT7 on the expression of neural cadherin (N-cadherin), Vimentin, E-cadherin, transforming growth factor-β (TGF-β), Ki-67, and Smad3 protein in glioma cells. Nude mouse tumor-bearing experiments were conducted to observe the effect of SIRT7 knockdown on glioma growth.ResultsHigher expression levels of SIRT7 gene were associated with poorer clinical prognosis (P<0.0001). SIRT7 expression levels were significantly correlated with tumor grading and 1p19q coding status (P<0.01). Compared with normal HA cells, glioma cells showed significantly increased SIRT7 expression levels (P<0.01). CCK-8 assay results indicated that, compared with control group, the proliferation activity of glioma cells in SIRT7 knockout group was significantly decreased (P<0.01), while SIRT7 overexpression group showed significantly increased proliferation activity (P<0.01). EdU assay results showed that, compared with control group, the proportion of glioma cells in the proliferative stage was significantly decreased in SIRT7 knockdown group (P<0.01), and significantly increased in SIRT7 overexpression group (P<0.01). Western blotting results revealed that, compared with control group, the protein expression levels of TGF-β, Smad3, N-cadherin and Vimentin were significantly decreased in SIRT7 knockdown group (P<0.01), while the expression level of E-cadherin protein was significantly increased (P<0.05). SIRT7 overexpression group showed significantly increased protein expression levels of TGF‑β, Smad3,N-cadherin and Vimentin (P<0.05), and a significantly decrease in E-cadherin protein expression level (P<0.05). Scratch assay results indicated that, compared with control group, the migration ability of cells in SIRT7 knockdown group and drug group was significantly decreased (P<0.01), and SIRT7 overexpression group showed significantly increased cell migration ability (P<0.05). Compared with drug group, drug+SIRT7 overexpression group exhibited significantly increased cell migration ability (P<0.01). Transwell assay results showed that, compared with control group, the migration and invasion abilities of cells in SIRT7 knockdown group and drug group were significantly decreased (P<0.01), and SIRT7 overexpression group exhibited significantly increased migration and invasion abilities (P<0.01). Compared with drug group, drug+SIRT7 overexpression group showed significantly increased migration and invasion abilities (P<0.01). Nude mouse tumor-bearing assay results indicated that the volume and weight of glioma in SIRT7 knockdown group were significantly reduced compared with control group (P<0.01).ConclusionsGlioma patients with high SIRT7 expression have poorer clinical prognosis. SIRT7 can regulate the TGF-β/Smad3 pathway to mediate EMT, promoting the proliferation and migration of glioma cells. SIRT7 knockdown can inhibit the growth of transplanted gliomas in nude mice.关键词：glioma;sirtuins7;epithelial-mesenchymal transition;transforming growth factor-β;Smad311|0|0发布时间：2025-01-23 - “最新研究发现，丹皮酚能缓解A53T-α-Syn突变诱导的神经细胞损伤，通过激活自噬途径清除过表达的α-Syn蛋白，其机制可能与上调JNK/Bcl-2介导的自噬途径有关。”
摘要：ObjectiveTo investigate the protective effects of paeonol (PAE) on autophagy in human neuroblastoma cells (SH-SY5Y) induced by overexpression of α-synuclein (α-Syn), and to explore its related mechanism.MethodsSH-SY5Y cells served as control group, while those induced with A53T-α-Syn mutation were used as model group. Additional groups included PAE (150 μg/ml) group, 3-MA (1 mmol/L) group, and PAE(150 μg/ml)+3-MA (1 mmol/L) group. Cell viability was assessed using CCK-8 method, cell morphology was observed under an optical microscope, and protein expressions of α-Syn, LC3-Ⅱ, p62, Beclin-1, phosphorylated c-Jun N-terminal kinase (p-JNK), and p-Bcl-2 were determined by Western blotting.ResultsCompared with control group, model control exhibited decreased cell survival (P<0.01), increased α-Syn expression (P<0.001), reduced expression of autophagy-related proteins LC3-Ⅱ and Beclin-1 (P<0.01, P<0.05), elevated autophagy substrate protein p62 (P<0.05), and decreased expression of autophagy pathway-related proteins p-JNK and Bcl-2 (P<0.05, P<0.01). Compared with model group, PAE group showed increased cell survival (P<0.01), decreased α‑Syn and p62 protein expression (P<0.01, P<0.05), and increased expression of LC3-Ⅱ, Beclin-1, p-JNK and Bcl-2 (P<0.05). Compared with PAE group, 3-MA+PAE group demonstrated increased α-Syn expression (P<0.05).ConclusionsPAE could attenuate the injury of SH-SY5Y cells induced by A53T-α-Syn and eliminate over-expressed α‑Syn by activating autophagy pathway, which may be associated with the upregulation of JNK/Bcl-2 mediated autophagy pathway.关键词：paeonol;Parkinson's disease;α-synuclein;autophagy10|0|0发布时间：2025-01-23 - “最新研究发现，miR-373-3p在糖尿病视网膜病变患者血清中低表达，可能通过靶向VEGFA抑制高糖诱导的视网膜微血管内皮细胞功能障碍。”
摘要：ObjectiveTo investigate the effect of miR-373-3p in diabetic retinopathy (DR), as well as the underlying mechanisms.MethodsSerum samples from 35 DR patients and 35 non-DR patients visiting Tianjin Fifth Central Hospital from February 2021 to February 2022 were collected, and expression levels of miR-373-3p and vascular endothelial growth factor A (VEGFA) mRNA were detected using quantitative reverse transcription polymerase chain reaction (qRT-PCR). An in vitro DR model was constructed using high glucose (HG)-treated human retinal microvascular endothelial cells (HRMEC). HRMECs were divided into control group (5 mmol/L glucose and 25 mmol/L mannitol treatment), HG group (30 mmol/L glucose treatment), HG+miR-373-3p mimic-negative control (miR-con) group (30 mmol/L glucose treatment after transfection with miR-con), HG+miR-373-3p mimic group (30 mmol/L glucose treatment after transfection with miR-373-3p), HG+miR-373-3p+vector group (30 mmol/L glucose treatment after co-transfection with miR-373-3p and vector), and HG+miR-373-3p+vascular endothelial growth factor A (VEGFA) group (30 mmol/L glucose treatment after co-transfection with miR-373-3p and VEGFA). The expression levels of miR-373-3p, VEGFA mRNA and protein were analyzed by qRT-PCR and Western blotting. CCK-8, immunofluorescence, Transwell assay, angiogenesis assay, and Western blotting were used to evaluate HRMEC proliferation, migration and angiogenesis abilities. The relationship between miR-373-3p and VEGFA was determined by dual luciferase reporter assay.ResultsCompared with non-DR patients, DR patients exhibited significantly lower expression levels of miR-373-3p (P<0.05) and higher expression levels of VEGFA mRNA (P<0.05) in serum. Compared with control group, HG group showed decreased expression of miR-373-3p (P<0.05), increased expressions of the mRNA and protein of VEGFA (P<0.05), higher cell viability, proliferation rate, proliferating cell nuclear antigen (PCNA) and Cylin D1 protein, and numbers of migrating cells and angiogenesis ability (P<0.05) in HRMECs. Compared with HG+miR-con group, HG+miR-373-3p group showed increased expression of miR-373-3p (P<0.05), decreased expressions of VEGFA (P<0.05), lower cell viability, proliferation rate, PCNA and Cylin D1 protein (P<0.05), and lower numbers of migrating cells and angiogenesis ability (P<0.05) in HRMECs. Compared with HG+miR-373-3p+vector group, HG+miR-373-3p+VEGFA group showed increased expression of VEGFA (P<0.05), higher cell viability, proliferation rate, PCNA and Cylin D1 protein, and numbers of migrating cells and angiogenesis ability (P<0.05) in HRMECs. The results of dual luciferase reporter assay showed decreased enzymatic activity of luciferase after cotransfection of miR-373-3p and VEGFA sequence (P<0.05).ConclusionMiR-373-3p is lowly expressed in the serum of DR patients, and its potential mechanism may involve targeting VEGFA to inhibit HG-induced HRMEC dysfunction.关键词：miR-373-3p;diabetic retinopathy;vascular endothelial growth factor A;high glucose;retinal microvascular endothelial cells14|0|0发布时间：2025-01-23
Basic Research
- “高尔基体应激反应研究进展，为感染性疾病防治提供新策略。”
摘要：The Golgi apparatus (GA), an essential membrane organelle in eukaryotes positioned between the endoplasmic reticulum and the plasma membrane, is responsible for transporting and modifying proteins and lipids generated by the endoplasmic reticulum. The homeostasis of the endoplasmic reticulum and GA plays a crucial role in regulating cellular life. When GA is unable to bear the load of protein or lipid processing and transportation, it enters a state of stress. Cells sense this and activate Golgi-related quality control mechanisms. By regulating the structure and function of GA, it provides important protection for the cell or induces programmed cell death, known as the GA stress response. Understanding the mechanisms and outcomes of cellular GA stress is of great significance for exploring Golgi dynamics and its impact on human diseases. This review summarizes the structure and function of GA under physiological conditions, the phenomenon of GA stress under pathological conditions, and the role of GA in infectious diseases, aiming to provide a basis for the clinical study of new strategies for the prevention and treatment of infectious diseases.关键词：Golgi apparatus;Golgi apparatus stress;Golgi apparatus stress response;infectious diseases14|0|0发布时间：2025-01-23 - “在心血管领域，心源性休克治疗取得进展，机械循环辅助降低病死率。专家综述了国内外MCS装置最新研究，为临床医师提供指导。”摘要：Cardiogenic shock (CS) is a disease characterized by reduced cardiac output leading to inadequate end-organ perfusion and remains a significant issue in cardiovascular medicine. Despite significant therapeutic advancements in recent years, the mortality rate associated with CS remains high. Mechanical circulatory support (MCS) has emerged as an innovative therapeutic strategy, capable of maintaining hemodynamic stability and ensuring systemic perfusion of vital organs, thereby effectively improving patient prognosis and lowering mortality rates. This review summarizes the latest research progress on commonly used MCS devices, both domestically and internationally, covering aspects of CS overview, left and right heart assist, bicardial and total heart assist, as well as the selection and limitations of MCS devices. We provide an overview of the types of MCS devices currently available and their indications for use, aiming to offer better clinical guidance to physicians and enhance the treatment level of CS.关键词：cardiogenic shock;mechanical circulatory support;Acute myocardial infarction10|0|0发布时间：2025-01-23
- “纳米颗粒在椎间盘退变治疗领域的研究进展，为临床转化提供参考。”
摘要：Intervertebral disc degeneration (IDD) is a prevalent clinical degenerative disease that currently can only be treated through conservative and surgical treatments, which only alleviate symptoms and are not significantly effective. In recent years, nanoparticles have been widely studied in the biomedical field due to their biodegradability, biocompatibility, extended body circulation, sustained and controlled release, and precise drug targeting. Nanoparticle drug delivery systems have the potential to deliver a range of therapeutic agents including proteins, drugs, genes, and cells, thereby promoting tissue and cell repair and regeneration, which offers hope for IDD treatment. However, there are still challenges in translating experimental data into practical therapies applicable to humans. This review summarizes recent research progress on drug delivery systems for IDD treatment based on nanoparticles and provides insights and prospects for the challenges faced by nanoparticles, aiming to provide a reference for the clinical translation of nanoparticle-based treatment for IDD.关键词：intervertebral disc degeneration;nanoparticles;drug delivery;carrier;targeting13|0|0发布时间：2025-01-23 - “最新研究发现，皮脂腺类器官为研究皮脂腺相关疾病提供理想平台，为发病机制和药物治疗研究提供参考。”
摘要：Sebaceous glands are vital appendages of the skin, primarily functioning in the secretion of a variety of lipid substances. These lipid substances play crucial physiological roles in the body, such as participating in body temperature regulation, maintaining skin homeostasis, and modulating the immune system. Abnormalities in the function of sebaceous glands can lead to a range of skin disorders, with acne being the most common one. Acne arises from the overproduction of sebum by sebaceous gland, leading to hair follicle blockage, bacterial infection, and inflammation response. Additionally, sebaceous gland carcinoma is a rare but severe malignant tumor of the skin, and its exact mechanisms are not fully understood. Organoids are closely resemble in vivo counterparts in terms of cell types, spatial structure, and function. Sebaceous gland organoids serve as an ideal platform for studying sebaceous glands and their associated diseases. This review summarizes the structure, function, homeostasis of sebaceous glands, as well as the construction and applications of sebaceous gland organoids, aiming to provide reference for research on the pathogenesis and treatment of sebaceous gland related diseases.关键词：skin;sebaceous gland;organoid;application11|0|0发布时间：2025-01-23
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