最新刊期
卷 49 ， 期 1 ， 2024
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Characteristics and development trend of war injury treatment under joint all-area combat situation AI导读
摘要：At the beginning of this century, breakthroughs and innovations in disruptive technologies represented by artificial intelligence have profoundly affected the development of human society and have become a central component in leading the new global military transformation. Saving the lives of the wounded and technological innovation are the driving force behind the development of military medicine. How to fight a battle and how to save people is the question that military medicine must answer. Joint all-area combat is a new military concept, representing the development direction of the future combat style, it has a strong strategic guiding significance for the future war and also puts forward higher requirements for the treatment of war injuries. "Platinum ten minutes, gold one hour" is the fundamental law of the battlefield first-line time-saving treatment, the war injury treatment must serve in the joint all-area combat demand and service requirements. This paper discusses the issue of demand or war injury treatment capacity building under the joint all-area combat pasture for criticism and correction.关键词：combat casualty care;joint all-area combat;revolution in military affairs;military medicine279|539|1更新时间：2024-02-08
Academician Forum
- 摘要：Epilepsy is a common neurological disease, has the characteristics of recurrent attacks and long-term treatment, thus bringing great pressure to patients and their families. Therefore, it is particularly important to do a good job of disability assessment. In recent years, with the development of the discipline, academic organizations such as the International League Against Epilepsy (ILAE) and China Association Against Epilepsy (CAAE) have successively updated the definition and diagnostic criteria of epilepsy and seizures. However, some items of epilepsy in the current Criteria for Disability Rating of Military Personnel (Trial) issued by People's Liberation Army (PLA) in 2011 can no longer meet the latest guidelines at home and abroad. Therefore, we suggest that the items related to epilepsy in the Criteria for Disability Rating of Military Personnel (Trial) should be revised to ensure that the disability evaluation being completed fairly and successfully.关键词：epilepsy;electroencephalogram;military personnel;disability evaluation58|136|0更新时间：2024-02-08
Expert Review
- 摘要：Based on the background of a multidisciplinary treatment team and the increasing high-quality life aspirations of patients, the preservation of anal function for patients with low rectal cancer has undergone changes in recent years. With the optimization of neoadjuvant therapy, refinement of surgical techniques, and the deepening of the concept of anal preservation after surgery, the concept of anal preservation for low rectal cancer has gradually shifted from traditional simple surgery to comprehensive treatment, and anal preservation surgery tends to be more accurate preservation. The goal of comprehensive treatment is to preserve good anal function and reduce surgical damage. However, comprehensive treatment for anal preservation in low rectal cancer is still in its infancy, and there is no consensus on the strategy planning for anal preservation. Therefore, summarizing various preoperative, intraoperative, and postoperative treatment strategies for low rectal cancer is of great significance for the selection of anal preservation schemes for patients with low rectal cancer. This article focus on exploring the optimization of neoadjuvant therapy models, "watch and wait" plans, the development of anal preservation techniques, and postoperative and preservation strategies, aiming to review the current status of anal preservation strategy planning for low rectal cancer.关键词：low rectal cancer;anal preservation surgery;neoadjuvant therapy;watch and wait64|293|0更新时间：2024-02-08
- 摘要：Diagnosis and treatment of locally advanced rectal cancer (LARC) must be completed by a collaborative model of a multi-disciplinary team. The neoadjuvant chemoradiotherapy significantly reduced the local recurrence rate of LARC, but did not affect the occurrence of distant metastases and overall survival. Total neoadjuvant therapy (TNT), by strengthening the intensity of chemotherapy and extending the time from radiotherapy to surgery, can improve the tumor response rate as well as disease-free survival rate and metastasis-free survival rate. It offers advantages such as enhancing the compliance with chemotherapy, maximizing tumor regression, improving survival and increasing the chance of organ preservation. TNT is a promising treatment model for LARC patients with high risk of distant metastasis or strong desire for organ preservation. With the application of immunotherapy in the field of TNT, the mode of TNT continues to expand. And the exploration of therapeutic predictive markers will help to provide a personalized treatment for patients.关键词：locally advanced rectal cancer;total neoadjuvant therapy;neoadjuvant chemoradiotherapy;disease-free survival;organ preservation55|342|0更新时间：2024-02-08
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摘要：ObjectiveTo identify the clinical characteristics and prognostic factors of young patients with sporadic rectal cancer liver metastasis (RCLM).MethodsThe clinical data of young RCLM patients at 45 years or under (n=40, as younger patient group) in Peking University First Hospital from January 2016 to January 2021 were reviewed, meanwhile, elder RCLM patient group were comprised of 82 patients older than 45-year-old in a 1:2 ratio. Proportions of categorical variables were compared between young patients and old patients. The clinicopathologic parameters were analyzed with univariate and multivariate Cox regression models and Kaplan-Meier method for demonstrating survival differences between the maximum diameter of liver metastasis and local therapy.ResultsOne hundred and twenty-two RCLM patients were identified, the 1-, 3- and 5-year survival rates of young patient group were 97.5%, 47.5%, 15.0%, those of elder patient group were 84.1%, 26.8%, 9.8%, respectively. The differences in BMI (P=0.008), primary tumor with obstruction and bleeding (P=0.006), synchronous rectal cancer liver metastases (P=0.005), the maximum diameter of liver metastasis >3 cm (P=0.019) were statistically significant between the two groups. And univariate and multivariate analyses showed that age (P=0.003), N stage (P=0.007), local therapy for liver metastases (P=0.047) and the maximum diameter of liver metastasis (P=0.030) were independent risk factors for influencing the prognosis of RCLM patients; curative resection or not of primary tumor (P=0.035) and the maximum diameter of liver metastasis (P=0.041) were independent risk factors for influencing the prognosis of young RCLM patients. Kaplan-Maier curve demonstrated survival differences between the maximum diameter of liver metastasis and local therapy for liver metastasis in RCLM patients (log-rank P=0.000).ConclusionsAlthough with later staging of initial tumor station, young RCLM patients may obtain better survival benefit compared with old patients. Higher degree of lymph node metastasis, local therapy for liver metastases and the maximum diameter of liver metastasis >3 cm indicates poor prognosis in RCLM patients, and without curative resection of primary tumor and maximum diameter of liver metastasis are also considered as the independent poor prognostic factors of young RCLM patients. Local therapy for liver metastases appears to play an important role in the treatment strategy of RCLM patients.关键词：rectal cancer liver metastasis;young patients;prognostic factors68|254|0更新时间：2024-02-08 -
摘要：ObjectiveTo compare the clinical efficacy of natural orifice specimen extraction surgery (NOSES) combined with enhanced recovery after surgery (ERAS) and conventional laparoscopic surgery in treatment of rectal cancer patients.MethodsA hundred patients with rectal cancer in the Department of Colorectal and Anal Surgery, the 940th Hospital of Joint Logistics Support Force of PLA were selected as the research objects from January 2019 to December 2021, including 49 cases treated with NOSES combined with ERAS were set as the observation group, and 51 cases treated with conventional laparoscopic surgery of rectal cancer were set as the control group. Postoperative C-reactive protein (CRP), procalcitonin (PCT), interleukin-6 (IL-6), white blood cell count (WBC), percentage of neutrophil (NEUT), K+ concentration, postoperative peristalsis recovery time, first time out-of-bed activity, first liquid feeding time, removal time of urinary, and drainage tubes, hospital stay and intraoperative blood loss, number of lymph node detection, positive margin rate, incidence of complications, and pain score were compared between the two groups.ResultsThe observation group showed significant lower postoperative CRP, PCT, IL-6 levels when compared with control group(P<0.05), while no significant difference in K+ concentration between the two groups (P>0.05). The recovery time of intestinal peristalsis, the time of the first out-of-bed activity, the first liquid feeding time, the time of removal of the urinary tube and the drainage tube, and the number of days of hospitalization in observation group were all superior to the control group (P<0.05). The observation group with the duration of surgery was longer than the control group, and the difference was statistically significant (P<0.05). There were no significant differences in intraoperative bleeding, number of lymph nodes detected and positive rate of resection margins between the two groups (P>0.05). There was no significant difference in the incidence of complications between the two groups (4.1% vs. 7.8%, P>0.05). The observation group showed significant lower pain scores than control group on the 1st, 2nd and 3rd day after surgery (P<0.05). ConclusionsNOSES combined with ERAS in treatment of rectal cancer patients is safe and feasible, can narrow the surgical incision, alleviate stress response and postoperative pain, improve postoperative efficacy and reduce the occurrence of complications, so is worthy of promotion and application.关键词：rectal cancer;natural orifice specimen extraction surgery;enhanced recovery after surgery;laparoscopy;surgery78|223|0更新时间：2024-02-08 -
摘要：ObjectiveTo evaluate the application value of hydrogel in image guided radiotherapy (IGRT) for prostate cancer (PCa).MethodsEighty PCa patients in the First Affiliated Hospital of Hebei North University from October 2022 to February 2023 were collected. The patients were divided into experiment group injected with hydrogel (n=33) and control group without hydrogel (n=47) by central random system allocation. The acute and advanced radiation proctitis (RP) incidence rate of the two groups were compared. Patients in experimental group were divided into RP group (n=5) and non-RP group (n=28). The independent risk factors leading to RP were analyzed by using logistic regression for PCa patients.ResultsIn experiment group, 12.1% (4/33) of the patients developed acute grade Ⅰ RP, and 3.0% (1/33) developed advanced grade Ⅰ RP. In control group, 31.9% (15/47) of the patients developed acute grade Ⅰ RP, and 12.8% (6/47) developed acute grade Ⅱ RP; 19.1% (9/47) of patients developed advanced grade Ⅰ RP, 4.3% (2/47) developed advanced grade Ⅱ RP, and 2.1% (1/47) developed advanced grade Ⅲ RP. The incidence of acute and advanced RP in experiment group was lower than that in control group (P<0.05). The application of hydrogel effectively reduced rectal toxicity. Age, rectal volume, V70 and V78 were independent risk factors for the incidence of RP in PCa patients (P<0.05). The characteristics of hydrogel injection were not related to the incidence of RP for PCa patients.ConclusionsHydrogel can effectively reduce the rectal toxicity for PCa patients in IGRT and has little impact on the overall treatment. Hydrogel has certain clinical application and promotion value.关键词：prostate cancer;image guided radiotherapy;hydrogel;application value46|113|0更新时间：2024-02-08
Special Issue on Rectal Cancer Surgery and Perioperative Treatment Strategies
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摘要：ObjectiveTo summarize the epidemiological and clinical features of infectious diseases of the central nervous system (CNS) by a single-center analysis.MethodsA retrospective analysis was conducted on the data of 1247 cases of CNS infectious diseases diagnosed and treated in the First Medical Center of PLA General Hospital from 2001 to 2020.ResultsThe data for this group of CNS infectious diseases by disease type in descending order of number of cases were viruses 743(59.6%), Mycobacterium tuberculosis 249(20.0%), other bacteria 150(12.0%), fungi 68(5.5%), parasites 18(1.4%), Treponema pallidum18(1.4%) and rickettsia 1(0.1%). The number of cases increased by 177 cases (33.1%) in the latter 10 years compared to the previous 10 years (P<0.05). No significant difference in seasonal distribution pattern of data between disease types (P>0.05). Male to female ratio is 1.87︰1, mostly under 60 years of age.Viruses are more likely to infect students, most often at university/college level and above, farmers are overrepresented among bacteria and Mycobacterium tuberculosis, and more infections of Treponema pallidum in workers. CNS infectious diseases are characterized by fever, headache and signs of meningeal irritation, with the adductor nerve being the more commonly involved cranial nerve. Matagenomic next-generation sequencing improves clinical diagnostic capabilities. The median hospital days for CNS infectious diseases are 18.00(11.00, 27.00) and median hospital costs are -
摘要：ObjectiveTo investigate the association between body mass index (BMI), sex hormone and single nucleotide polymorphisms (SNPs) of follicle-stimulating hormone receptor (FSHR) gene rs2268361 and rs2349415 and its correlation with the risk of polycystic ovary syndrome (PCOS).MethodsPeripheral blood was collected from 213 PCOS patients and 207 healthy controls, attending the Department of Reproductive Medicine at the First Hospital of Shanxi Medical University, and 32 follicular fluids were randomly collected from each of the PCOS and control groups from March to August 2021. Calculation of BMI of the PCOS and control groups; The levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2), testosterone (T), progesterone (P) and prolactin (PRL) in peripheral blood of the two groups were detected by immunochemiluminescence method. Polymerase chain reaction (PCR) and high-resolution melting curve (HRM) were used to analyze the polymorphisms of rs2268361 and rs2349415 in FSHR of the two groups. Quantitative real-time PCR was used to detect the expression of FSHR gene mRNA in peripheral blood and ovarian granulosa cells.ResultsThere was a strong positive correlation between LH and LH/FSH(r=0.88, P<0.05); The levels of BMI, E2, LH, LH/FSH and T in PCOS group were significantly higher than those in control group(P<0.05); FSH level was significantly lower than that of control group (P<0.001). HRM analysis showed the frequencies of CC, CT and TT genotypes at rs2349415 were 55.9%, 34.3% and 9.8% in PCOS group and 68.6%, 23.2% and 8.2% in control group, respectively. The frequencies of C and T alleles were 73.0% and 27.0% in PCOS group and 80.2% and 19.8% in control group, respectively. There were significant differences in genotype frequencies and allele frequencies between the two groups (P<0.05); The expression level of FSHR mRNA was higher in ovarian granulosa cells in PCOS group than in control group (P=0.004), the expression level of FSHR mRNA in rs2349415 TT genotype was higher than that in CC (P=0.002) and CT (P=0.035) genotype. ConclusionHigh levels of BMI, LH, E2 and T allele of rs2349415 increased the risk of PCOS.关键词：polycystic ovary syndrome;follicle stimulating hormone receptor;single nucleotide polymorphism;sex hormones54|276|0更新时间：2024-02-08 -
Prognostic significance of
MyD88L265P andCD79B mutations in primary central nervous system lymphoma AI导读
摘要：ObjectiveTo analyze the relationship between MyD88L265P and CD79B mutations in tumor tissue and the prognosis of primary central nervous system lymphoma (PCNSL).Methods18 PCNSL patients with normal immune function (no history of HIV infection and immunosuppressants administration) who were diagnosed by craniotomy or stereotaxic biopsy in the Second Hospital of Lanzhou University from August 2018 to November 2020 were retrospectively analyzed. Real-time quantitative PCR and first-generation sequencing techniques were respectively used to detect MyD88L265P and CD79B mutations in tumor tissues of 18 PCNSL patients. Univariate analysis and Cox regression multivariate analysis were performed for indicators that may be associated with first progression-free survival (PFS) and overall survival in PCNSL.ResultsThe mutation rate of MyD88L265P was 38.9%, the mutation rate of CD79B was 33.3%, and the co-mutation rate of MyD88L265P/CD79B was 27.8% in PCNSL tissue of 18 patients. Univariate analysis showed that the PCNSL patients with multiple lesions, deep involvement of lesions, and tissue CD79B mutation had a statistically significant shorter time of PFS (P<0.05). Multivariate analysis showed that deep lesion involvement(HR=0.135, 95%CI 0.023-0.799, P<0.05) and CD79B mutation (HR=0.149, 95%CI 0.028-0.800, P<0.05) in PCNSL tissue were independent prognostic factors for PCNSL patients.ConclusionThe frequency of MyD88L265P and CD79B mutations was high in tumor tissues of 18 PCNSL patients, and these two gene mutations may be associated with poor prognosis of PCNSL, especially CD79B mutation.关键词：primary central nervous system lymphoma;MyD88 mutation;CD79B mutation;prognosis48|169|0更新时间：2024-02-08 -
摘要：ObjectiveTo investigate the effect of antinuclear antibodies (ANAs) on hormone response in patients with autoimmune hepatitis (AIH)-primary biliary cholangitis (PBC) overlap syndrome (AIH-PBC OS) and AIH-only within half a year.MethodsA retrospective analysis of 77 patients with autoimmune liver disease (AILD) admitted to First Clinical Medical College of Lanzhou University from January 2018 to December 2021, all of whom were confirmed by liver biopsy and receiving glucocorticoid treatment. Among them, 46 patients were in AIH-PBC OS group and 31 were in AIH-only group. The general clinical characteristics, liver puncture-related indexes, autoantibodies and immunoglobulin indexes of patients in each group at the time of diagnosis were collected and compared, and the biochemical and immunoglobulin indexes of patients at the time of hormone use and at the time of review within 6 months were also collected, and the hormone response within 6 months was evaluated according to the levels of glutamic transaminase (AST), glutamic alanine transaminase (ALT) and immunoglobulin G (IgG), and the effect of ANAs on hormone response outcomes in both groups over a six-month period was analyzed. Multifactorial ordered logistic analysis was performed to evaluate the effect of ANAs on hormone response between two groups.ResultsThere was no statistically significant difference in the percentage of AIH-PBC OS and AIH-only patients among both ANAs-positive and -negative AILD patients (55.6% vs. 44.4% and 65.6% vs. 34.4%, P>0.05). Among 46 patients with AIH-PBC OS, there were 25 in ANAs-positive group and 21 in ANAs-negative group. The rate of complete hormone response within 6 months was lower than that of ANAs-negative group (44.0% vs. 76.2%), while the rate of hormone non-response was higher than that of ANAs-negative group (20.0% vs. 0), and the difference was statistically significant (P<0.05). There were 20 cases of ANAs-positive and 11 cases of ANAs-negative in the 31 AIH-only patients. There was no statistically significant difference in the results of hormone response within 6 months between the two groups (P>0.05). Multifactorial ordered logistic analysis showed that AIH-PBC OS patients were more likely to have a higher likelihood of 6-month hormone non-response rate in ANAs-positive patients, and the difference was statistically different (P<0.05). And there was no significant effect of ANAs type on hormone response outcome in AIH-only patients (P>0.05).ConclusionAIH-PBC OS ANAs-positive patients have a poor hormone response within half a year. In AIH-only patients, ANAs have no significant effect on hormone response results.关键词：autoimmune hepatitis;primary biliary cholangitis;overlap syndrome;antinuclear antibodies;hormone biochemical response46|109|0更新时间：2024-02-08 -
摘要：ObjectiveTo investigate the value of magnetic resonance imaging (MRI) T2-mapping in evaluating the activity of Graves ophthalmopathy (GO).MethodsA total of 64 patients with GO in the Department of Endocrinology, the First Affiliated Hospital of Chongqing Medical University from July 2019 to January 2021 were collected. Simple random grouping was performed by computer, with 49 cases as observation subjects, and 15 patients for diagnostic test. According to clinical activity score (CAS), 49 GO patients were divided into active group (CAS ≥3 points, 48 eyes) and inactive group (CAS <3 points, 50 eyes). Normal control group (NC group) included 31 patients (62 eyes). All subjects underwent 3.0T orbital MRI T2-mapping. Measuring the T2 relaxation time (T2RT) of superior rectus, inferior rectus, medial rectus, and lateral rectus on five layers behind the eyeball on T2-mapping coronal images, and select the maximum value of T2RT in the five layers for each extraocular muscle to represent the T2RT of this extraocular muscle. Finally, select the maximum T2RT values of the four extraocular muscles, expressed as extraocular muscle maximum T2RT. Compare the differences of the above 5 indicators (superior rectus T2RT, inferior rectus T2RT, medial rectus T2RT, lateral rectus T2RT, extraocular muscle maximum T2RT) between active group, inactive group and NC group. ROC curve was used to analyze the diagnostic value of the above 5 indicators for GO activity assessment, and the diagnostic threshold was obtained. Then, another 15 GO patients were performed for diagnostic tests evaluation to determine the indicators of high diagnostic efficacy and the threshold of diagnostic activity.ResultsThe T2RT of all extraocular muscles in active group were significantly higher than those in inactive group and NC group, the difference was statistically significant (P<0.001). The threshold value of the five indicators were obtained by ROC curve analysis. The maximum T2RT cut-off values of superior rectus muscle, inferior rectus muscle, medial rectus muscle, lateral rectus muscle and extraocular muscles for judging activity were 80.200 ms, 97.045 ms, 94.355 ms, 85.750 ms and 101.385 ms respectively. Another 15 GO patients were performed for diagnostic tests, the indexes with relatively high sensitivity, specificity, positive predictive value and negative predictive value were inferior rectus T2RT and extraocular muscle maximum T2RT, the cut-off values of GO activity were 97.045 ms and 101.385 ms, respectively; the sensitivity were 91.7% and 93.8%, respectively; the specificity all were 80.0%.ConclusionsMRI T2-mapping sequence has a good value in assessment of GO activity. The inferior rectus T2RT and extraocular muscle maximum T2RT can be choosed to evaluate the activity of GO.关键词：Graves ophthalmopathy;magnetic resonance imaging T2-mapping;activity assessment42|132|0更新时间：2024-02-08 -
摘要：ObjectiveTo explore the efficacy and safety of ticagrelor de-escalation and nicorandil therapy in elderly patients with acute coronary syndrome (ACS) after percutaneous coronary intervention (PCI).MethodsA total of 300 elderly patients with ACS were selected from the Sixth and Seventh Medical Center of Chinese PLA General Hospital and Beijing Chaoyang Integrative Medicine Emergency Rescue and First Aid Hospital from November 2016 to June 2019, including 153 males and 147 females, aged>65 years old. All the patients received PCI, and all had double antiplatelet therapy (DAPT) scores ≥2 and a new DAPT (PRECISE-DAPT) score of ≥25. All patients were divided into two groups by random number table method before operation: ticagrelor group(n=146, ticagrelor 180 mg load dose followed by PCI, and ticagrelor 90 mg bid after surgery) and ticagrelor de-escalation + nicorandil group (n=154, ticagrelor 180 mg load dose followed by PCI, ticagrelor 90 mg bid+nicorandil 5 mg tid after surgery, changed to ticagrelor 60 mg bid+ nicorandil 5 mg tid 6 months later). Follow-up was 12 months. The composite end points of cardiovascular death, myocardial infarction and stroke, the composite end points of mild hemorrhage, minor hemorrhage, other major hemorrhage and major fatal/life-threatening hemorrhage as defined by the PLATO study, and the composite end points of cardiovascular death, myocardial infarction, stroke and bleeding within 12 months in the two groups were observed. ResultsThe comparison of general baseline data between the two groups showed no statistically significant difference (P>0.05). There was also no significant difference in the composite end points of cardiovascular death, myocardial infarction and stroke between the two groups (P>0.05). The cumulative incidence of bleeding events in ticagrelor de-escalation + nicorandil group was significantly lower than that in ticagrelor group (P<0.05), while the composite end points of cardiovascular death, myocardial infarction, stroke and bleeding were also significantly lower than those in tecagrelor group (P<0.05).ConclusionIn elderly patients with ACS, the treatment of ticagrelor de-escalation + nicorandil after PCI may not increase the incidence of ischemic events such as cardiovascular death, myocardial infarction or stroke, and it may reduce the incidence of hemorrhagic events.关键词：acute coronary syndrome;percutaneous coronary intervention;ticagrelor;nicorandil;de-escalation therapy53|215|0更新时间：2024-02-08
Clinical Research
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摘要：ObjectiveTo observe the effects of amyloid-β (Aβ) receptor PirB on mouse astrocyte proliferation and reactive astrogliosis in vitro.MethodsMouse primary astrocytes were cultured, and divided into control group, Aβ group, Aβ+0.2 μmol/L PEP group, Aβ+0.4 μmol/L PEP group, Aβ+Fluspirilene group, Aβ+GFP-LV group, and Aβ+mPirB-LV group. The mouse astrocytes were treated with soluble PirB extracellular peptide PEP or PirB inhibitor Fluspirilene, respectively, to inhibit endogenous PirB receptor, or overexpressed PirB gene via lentivirus transfection and then treated with Aβ1-42 oligomers. The proliferation of astrocytes was observed by RTCA and EdU methods, and the mRNA expression levels of S-100 calcium-binding protein B (S-100β), Vimentin, Nestin and amyloid precursor protein (APP) associated with reactive astrogliosis of astrocytes were observed by real-time PCR, and the expression level of glial fibrillary acid protein (GFAP) was detected by Western-blotting.ResultsThe results of RTCA monitoring showed that normalized cell index (NCI) values of each group decreased sharply after treatment, and then increased gradually and tended to be stable. The results of EdU staining showed that the proliferative activity of astrocytes was significantly enhanced in the Aβ group (P<0.05) compared with control group; Compared with Aβ group, cell proliferation activity in Aβ+0.2 μmol/L PEP group, Aβ+0.4 μmol/L PEP group and Aβ+Fluspirilene group were significantly decreased (P<0.01 or P<0.001). The results of real-time PCR showed that compared with control group, mRNA expressions of GFAP, S-100β, Vimentin, Nestin, APP and PirB in Aβ group were significantly increased (P<0.05); Compared with Aβ group, mRNA expressions of GFAP, S-100β, Vimentin, Nestin, APP and PirB in Aβ+0.4 μmol/L PEP group were significantly decreased (P<0.01); Compared with Aβ+GFP-LV group, mRNA expressions of GFAP, S-100β, Vimentin, Nestin, APP and PirB in Aβ+mPirB-LV group were significantly increased(P<0.05). The results of Western blotting showed that compared with control group, the expression of GFAP in Aβ group was significantly increased (P<0.05); Compared with Aβ group, the expression of GFAP in Aβ+0.4 μmol/L PEP group was significantly decreased (P<0.05). ConclusionsPirB is an upstream molecule which could regulate astrocyte proliferation and reactive astrogliosis, and inhibiting PirB receptor in astrocytes may be a potential treatment for Alzheimer's disease.关键词：Alzheimer's disease;reactive astrogliosis;PirB;astrocytes;proliferation79|420|0更新时间：2024-02-08 -
摘要：ObjectiveTo investigate the effect and mechanism of pomalidomide (POM) on airway inflammation and mucus hypersecretion in rats with chronic obstructive pulmonary disease (COPD).MethodsThirty-six SD rats were randomly divided into control group, model group and POM group, with 12 in each group, half male and half female. The COPD model was established by smoke exposure combined with Klebsiella pneumoniae infection in model group and POM group. The rats in POM group were treated with POM (0.5 mg/kg, once a day for 1 week). The lung function, lung tissue pathology, the proportion of inflammatory cells in bronchoalveolar lavage fluid (BALF) and the levels of serum inflammatory factors tumor necrosis factor-α (TNF‑α), interleukin (IL)‑1β, IL-6 and IL‑13 were observed and detected in each group. AB-PAS staining and immunohistochemistry were used to analyze the proliferation of goblet cells and the secretion of mucin (MUC) 5AC and MUC5B in airway epithelium of rats. The expression levels of TNF‑α receptor 1 (TNFR1), IκB kinase (IKK), phosphorylated IKK (p-IKK) and P65 protein in lung tissue were detected by Western blotting.ResultsCompared with control group, model group showed significant decreased of tidal volume (TV), minute ventilation (MV), forced expiratory vital capacity (FVC), 0.1s forced expiratory volume (FEV0.1) and 0.3 s forced expiratory volume (FEV0.3) (P<0.05), increased of the mean linear intercept (MLI) of the alveoli (P<0.01), decreased of the mean alveolar number (MAN) (P<0.01), increased of the proportion of neutrophils and lymphocytes in BALF sediment (P<0.05), and decreased of the proportion of macrophages in BALF sediment (P<0.01);increased of the levels of serum inflammatory factors TNF-α, IL-1β, IL-13 and IL-6 (P<0.05), the proportion of goblet cells in airway epithelium (P<0.01), the secretion of MUC5AC and MUC5B in lung tissue (P<0.01), the content of TNFR1 and the ratio of p-IKK / IKK (P<0.01), the content of P65 in nucleus (P<0.01); and decreased of the content of P65 in cytoplasm (P<0.05). Compared with model group, after one week of POM treatment, POM group showed significant improved of the TV, MV, FVC, FEV0.1, FEV0.3, MLI and MAN of rats (P<0.05); decreased of the proportion of neutrophils and lymphocytes in BALF (P<0.05); increased of the proportion of macrophages (P<0.01); decreased of the levels of serum TNF-α, IL-1β, IL-6 and IL-13 (P<0.05), the proportion of goblet cells in airway (P<0.01), the secretion of MUC5AC and MUC5B (P<0.01), and the expression of TNFR1, P-IKK and P65 (nucleus) (P<0.05); and increased of the level of P65 (cytoplasm) (P<0.01).ConclusionsPOM can improve airway inflammation and mucus hypersecretion in COPD rats, which may be achieved by inhibiting TNF-α/NF-κB signaling pathway.关键词：pomalidomide;chronic obstructive pulmonary disease;tumor necrosis factor-α;nuclear factor kappa-B;airway inflammation45|719|0更新时间：2024-02-08 -
摘要：ObjectiveTo investigate the efficacy of histone deacetylase (HDAC) inhibitor chidamide combined with the PD-1 inhibitor on CD8+ T cells anti-cancer function in OVA-expressing MC38 (MC38-OVA) colorectal-bearing mice.MethodsAnimal experiments: C57BL/6 tumor models were constructed by subcutaneously injecting MC38-OVA colorectal cancer cells into the back of mice. We randomized mice into control group, chidamide group, anti-PD-1 group and chidamide+anti-PD-1 group (20 each group). We monitored the tumor growth and animal survival rate of each group; we employed a flow-based method to detect the number and ratio of tumor-infiltrating CD8+ T cells, CD8+IFN-γ+ T cells, OVA antigen-specific CD8+ T cells, and the expression changes of regulatory T cells (Treg), myeloid-derived suppressor cells (MDSC), and tumor-associated macrophages (TAM). Cell experiments: We used a flow-based method to detect the apoptosis of CD8+ T cells and MC38-OVA tumor cells after 0, 10, 25, 50, 100, or 200 nmol/L chidamide treatment. The proliferation of CD8+ T cells and MC38-OVA tumor cells treated with 0 and 100 nmol/L chidamide was detected by Ki-67 antibody labeling and cell counting. To evaluate CD8+ T cell killing ability, we treated CD8+ T cells with various conditions (control group, chidamide group, anti-PD-1 group and chidamide+anti-PD-1 group) followed by co-culture with MC38-OVA tumor cells, using the flow-based method. In the condition that CD8+ T cells treated with 0 and 100 nmol/L chidamide co-cultured with the same number of MC38-OVA tumor cells, the expression of CD107a was detected by flow cytometry.ResultsCompared with control group, the tumor growth was inhibited (P<0.05) while the survival rate was improved (P<0.01) in chidamide+anti-PD-1 group. The number of tumor-infiltrating CD8+ T cells was significantly higher in chidamide group, anti-PD-1 group and chidamide+anti-PD-1 group than that in control group (P<0.05). Nonetheless, the ratio and levels of CD8+IFN-γ+ and OVA antigen-specific CD8+ T cells were significantly higher in chidamide+anti-PD-1 group than those in other groups (P<0.05). The in vitro experiment results showed that chidamide could enhance the killing ability of CD8+ T cells and the expression of CD107a.ConclusionChidamide combined with PD-1 inhibitor significantly enhanced the number and function of tumor-infiltrating CD8+ T cells and increased antigen-specific CD8+ T cells, which will provide a theoretical and experimental basis for the combination of chidamide in clinical solid tumor immunotherapy.关键词：T lymphocyte;immune checkpoint inhibitors;programmed death receptor-1;chidamide49|604|0更新时间：2024-02-08
Basic Research
- 摘要：Helicobacter pylori (HP) infection is a Class Ⅰ carcinogen in gastric cancer, closely related to the occurrence of gastric cancer. Many studies have shown that HP eradication has a preventive effect on gastric cancer. However, 2.7%-6.1% of patients with early gastric cancer who have been eradicated after endoscopic submucosal dissection (ESD) can still develop metachronous gastric cancer (MGC), and the mechanism of its occurrence is still unclear. In this review, the atrophy of gastric mucosa and intestinal metaplasia cannot be completely reversed after HP eradication, the excessive proliferation of gastric mucosa epithelial cells, the accumulation of genetic abnormalities, the homeostasis imbalance of the epigenetic group, changes in immune microenvironment, the abnormality of stem cells in gastric mucosa, chromatin accessibility, and changes in chromosome remodeling were discussed in the mechanism of carcinogenesis caused by the above molecular changes after ESD and HP eradication in early gastric cancer.关键词：endoscopic submucosal dissection;Helicobacter pylori;eradication;metachronous gastric cancer;pathogenesis74|415|0更新时间：2024-02-08
- “在心血管系统领域，专家综述了CypA/CD147在新冠感染中的作用，为诊疗提供参考。”
摘要：Corona virus disease 2019 (COVID-19) epidemic has been effectively controlled, but its related complications still cannot be ignored, especially the cardiovascular circulatory system is the active site of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Angiotensin-converting enzyme 2 (ACE2) is a type Ⅰ transmembrane glycoprotein that is highly expressed in heart, kidney and testis. Spike protein of SARS-CoV-2 invades host cells by binding to the cell surface receptor ACE2. However, there are still many deficiencies in the clinical application of vaccines and drugs developed based on this target. As a molecular chaperone, cyclophilin A (CypA) promotes protein folding and T cell activation. CD147 is one of the most widely studied CypA receptors, and the interaction of CypA/CD147 plays an important role in the entry of SARS-CoV-2 into host cells. However, there are few reports on the invasion of SARS-CoV-2 into the cardiovascular system through the CypA/CD147 signaling pathway. Based on this, this article summarizes the previous research evidence and the research basis of the research group, and reviews the structure and function of CypA/CD147, the role of CypA/CD147 in cardiovascular disease, and the cardiovascular disease caused by SARS-CoV-2 targeting CypA/CD147 signal pathway, in order to provide reference for the diagnosis and treatment of the COVID-19 complicated with cardiovascular system diseases.关键词：cyclophilin A;extracellular matrix metalloproteinase inducer;severe acute respiratory syndrome coronavirus 2;cardiovascular system;cardiovascular diseases55|187|1更新时间：2024-02-08
Review
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