最新刊期
卷 49 ， 期 7 ， 2024
- “在高原官兵眼干燥症治疗领域，中部战区总医院医疗队验证了改良睑板腺按摩联合超声雾化及药物治疗的疗效与安全性，为高原地区眼干燥症治疗提供了安全有效的解决方案。”
摘要：ObjectiveTo explore the efficacy and safety of modified meibomian gland massage combined with ultrasonic atomization and drug therapy for dry eye in high-altitude military personnel.MethodsA total of 180 patients (360 eyes) with dry eye who were diagnosed and treated by the medical team of General Hospital of Central Theater Command of Chinese PLA from July to October 2022 in Linzhi, Tibet (average altitude of 3100 meters) were selected as the research subjects. Patients were divided into four block groups based on the course of the disease: <1 month, 1-3 months, 3-6 months, and ≥6 months, and each block group was randomly assigned to control group, traditional group and modified group by random number table method, with 60 cases in each group. Control group received routine treatment (artificial tear drops and atomization fumigation); on the basis of routine treatment, traditional group underwent traditional meibomian gland massage, and modified group underwent modified meibomian gland massage. After 3 months of treatment, the scores of symptoms and signs, total effective rate, duration of treatment and incidence of adverse events were compared among the 3 groups.ResultsIn the intra-group comparison before and after treatment, except for control group's symptom scores difference which was not statistically significant (P>0.05), all other scores decreased significantly(P<0.05). In the inter-group comparison after treatment, the scores of both traditional group and modified group were significantly better than those of control group (P<0.05), but no significant difference was observed in symptom and sign scores between traditional and modified groups (P>0.05). Compared with control group, the total effective rates of traditional group and modified group both significantly increased (P<0.01), but there was no significant difference in total effective rate between traditional and modified groups (P>0.05). The treatment time in modified group was significantly longer than that in control group (P<0.05), but significantly shorter than that in traditional group (P<0.05). The incidence of adverse events was significantly lower in modified group than that in traditional group (P<0.001).ConclusionIn high-altitude areas, modified meibomian gland massage combined with ultrasonic atomization and local drug therapy for dry eye is safe and effective, non-invasiveness and easy to perform, and suitable for promoting and application in military field training.关键词：ophthalmoxerosis;high altitude;troops;modified meibomian gland massage- 108
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发布时间：2024-08-12 - “最新研究显示，CT影像组学特征在肺泡蛋白沉积症诊断中具有重要价值，为临床诊断提供了新思路。”
摘要：ObjectiveTo investigate the diagnostic value of CT radiomics features in pulmonary alveolar proteinosis (PAP).MethodsThe general data and clinical characteristics of 24 patients with PAP in the Chinese PLA General Hospital from November 2008 to August 2022 were retrospectively collected and analyzed. Another 53 patients with other diffuse lung diseases except for PAP during the same period served as control group. The differences in the 10 conventional CT signs (semantic features) and 107 radiomics features between the two groups were compared. All patients were randomly divided into the training group (n=53) and the validation group (n=24) at a ratio of 7:3. CT semantic feature model, radiomics model and combined model to diagnose PAP were constructed in training group, and the diagnostic efficacy of models was compared using the receiver operating characteristic (ROC) curve in validation group. Decision curve analysis (DCA) was used to assess the value of models for practical clinical application. Radscore was calculated for the model with the highest diagnostic efficacy.ResultsA total of 24 patients with pathologically confirmed PAP were enrolled, with a male to female ratio of 3:1 and an average age of (44.6±15.2) years. The main clinical symptoms of patients with PAP included shortness of breath, cough, sputum and chest tightness. Compared with control group, the incidence of pleural effusion in PAP group was significantly lower (P<0.05), while no significant differences were observed in other CT features(P>0.05). The areas under the curve (AUC) of the semantic feature model for diagnosing PAP in training and validation group were 0.590 and 0.594, respectively, and in validation group, the accuracy, sensitivity, and specificity for diagnosis of PAP were 0.188, 1.000, and 0.188, respectively. The AUCs of the radiomics model in training group and validation group were 0.845 and 0.867, respectively, and in validation group, the accuracy, sensitivity, and specificity were 0.641, 0.938, and 0.703, respectively. The AUCs of the combined model in training group and validation group were 0.850 and 0.883, respectively, and in validation group, the accuracy, sensitivity, and specificity were 0.688, 0.750, and 0.938, respectively. The AUCs of the radiomics model and the combined model were significantly greater than that of the semantic feature model, but there was no significant difference in the AUCs between the first two models. The decision curve analysis showed that both the radiomics model and the combined model had high application value for predicting PAP.ConclusionCT radiomics shows higher clinical value in the diagnosis of PAP compared with conventional CT features.关键词：pulmonary alveolar proteinosis;CT;radiomics;diagnostic model- 48
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发布时间：2024-08-12 - “最新研究揭示，胃印戒细胞癌病理亚型影响患者预后，兰州大学第二医院团队发现，混合型病理亚型、T2-T4分期和CA125阳性是预后不良的独立危险因素。”
摘要：ObjectiveTo explore the clinicopathological characteristics, prognosis and influencing factors of different pathological subtypes of gastric signet ring cell carcinoma (GSRC).MethodsA retrospective analysis was performed on the clinical data of 232 patients with GSRC collected from January 2016 to December 2018 in Lanzhou University Second Hospital. According to the WHO classification criteria for GSRC, the patients were divided into pure gastric signet-ring cell carcinoma (pGSRC, n=36) and mixed gastric signet-ring cell carcinoma (mGSRC, n=196). The follow-up as of September 30, 2022, the survival analysis was done using Kaplan-Meier method, the univariate and multivariate Cox regression were performed to analyze the risk factors affecting the prognosis of GSRC patients.ResultsThe median survival time of pGSRC and mGSRC patients was 41.0(6.0-70.0) months and 24.0(2.0-74.0) months, respectively. Kaplan-Meier analysis showed that combination with diabetes, anemia, tumor diameter, nerve invasion, lymphovascular invasion, T stage, N stage, GSRC pathological subtype, CA125 and tumor diameter could affect the overall survival (OS) of patients with GSRC after radical gastrectomy (P<0.05), but Her-2, whether adjuvant chemotherapy or not and others elements had no significant effect on OS of GSRC patients after radical gastrectomy (P>0.05). Univariate Cox regression analysis showed that the combination with diabetes (P=0.031), anemia (P=0.028), tumor diameter >5 cm (P=0.009), nerve invasion (P=0.002), lymphovascular invasion (P=0.002), mGSRC pathological type (P=0.039), T2-T4 stage (P=0.001), N1-N4 stage (P=0.004), pTNM stage Ⅲ (P=0.044), the number of lymph node metastasis >30 (P=0.044) and CA125 positive (P=0.009) were related to the prognosis of patients with GSRC after radical gastrectomy. Multivariate Cox regression analysis showed that mGSRC pathological type (P=0.035), T2-T4 stage (P=0.003), CA125 positive (P=0.010) were independent risk factors for poor prognosis of patients with GSRC after radical gastrectomy.ConclusionCompared with pGSRC, patients with mGSRC at diagnosis have higher pTNM stages, more aggressive, and shorter median survival time. mGSRC pathological type, T2-T4 stage, and CA125 positive were all independent factors affecting the prognosis of patients with GSRC.关键词：signet ring cell carcinoma;pure gastric signet-ring cell carcinoma;mixed gastric signet-ring cell carcinoma;prognosis- 42
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发布时间：2024-08-12 - “在医疗领域，解放军总医院第四医学中心的研究人员对烧伤患者围手术期焦虑状态进行了深入研究。研究发现，性别、术前疼痛程度及术前焦虑状态是影响烧伤患者焦虑状态的关键因素。研究结果为改善烧伤患者的围手术期管理提供了重要参考。”
摘要：ObjectiveTo evaluate the perioperative anxiety state and analyze the influencing factors of burned patients.MethodsA total of 110 burned patients undergoing selective surgery under general anesthesia were included in the Fourth Medical Center of Chinese PLA General Hospital from February to August 2022. All patients were evaluated with self-rating anxiety scale (SAS), visual analogue scale-anxiety (VAS-a), visual analogue scale-pain (VAS-p), mini-mental state examination (MMSE), and Ramsay sedation score 1-day before and after operation. The patients' parameters were recorded including mean arterial pressure (MAP) and heart rate (HR) at admission (T0), before anesthesia induction (T1), 2 min after intubation (T2), 15 min after surgery (T3), during surgery (T4), at surgery end (T5), and immediately after leaving the operating room (T6). The occurrence and the influencing factors of perioperative anxiety in burn patients were analyzed using the univariate and multivariate logistic regression.ResultsThe incidence of preoperative and postoperative anxiety in burn patients was 29.1% and 22.3% respectively. Univariate logistic analysis showed that gender (P=0.002), burn time (P=0.046), burn area (P=0.005), burn site (P=0.035), and degree of preoperative pain (P=0.001) were related with preoperative anxiety status in burn patients; while burn time (P=0.030), burn area(P=0.001), burn site (P=0.016), degree of preoperative pain (P=0.021), and preoperative anxiety status (P<0.001) were related with postoperative anxiety state in burn patients. Multivariate logistic regression analysis showed that gender and degree of preoperative pain were the independent influencing factors of preoperative anxiety status in burn patients (P=0.002, 0.022), and preoperative anxiety status was the independent influencing factor of postoperative anxiety status in burn patients (P<0.001). Compared with the preoperative non-anxious patients (n=73), preoperative anxious patients (n=30) showed no significant difference in MAP at each time point (P>0.05), but HR was accelerated (P<0.05), and the dosage of sufentanil, remifentanil and propofol increased significantly during the operation (P<0.05).ConclusionsThe anxiety state of burn patients was significantly higher before operation than that after operation, and their consumption of anesthetic drugs during operation was higher, and there was no significant correlation with the type and number of operation. Gender, degree of preoperative pain and anxiety state were the independent influencing factors of perioperative anxiety state in burn patients. Early intervention against relevant factors will help patients recover quickly.关键词：burns;perioperative;anxiety;pain- 57
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发布时间：2024-08-12 - “兰州市城关区中老年人恶性肿瘤发病率上升，死亡率下降，研究揭示了相关危险因素和保护性因素。”
摘要：ObjectiveTo analyze the incidence and mortality of malignant tumors among the middle-aged and elderly population in Chengguan district of Lanzhou from 2011 to 2021, and to discuss the related risk factors.MethodsUsing the research data of REACTION in Lanzhou, an epidemiological survey was conducted through cluster sampling in three communities in Chengguan district of Lanzhou since April 2011. The target population was middle-aged and elderly residents over 40 years old. Two follow-up surveys were carried out in 2014－2016 and 2021 successively, and 6543 people with complete follow-up data were finally included. The incidence and mortality rates of malignant tumors were calculated, as well as their age standardized rates with reference to the age composition of Segi's world standard population. Multivariate Cox regression analysis was used to screen the risk factors affecting the incidence and mortality of malignant tumors.ResultsAfter an average follow-up of 10.6 years, 314 new cases of malignant tumors were found in middle-aged and elderly residents in Chengguan district of Lanzhou, with an incidence rate and age standardized incidence rate of 454.30/100 000 and 128.93/100 000, respectively. A total of 158 deaths were attributed to malignant tumors, with a mortality rate and age standardized rate of 228.41/100 000 and 607.9/100 000, respectively; The age standardized incidence rate and mortality rate of malignant tumors males were both higher than those females (P<0.05). During the follow-up period, the age standardized incidence rate of malignant tumors in the general population showed an significant upward trend (P<0.05), whereas the age standardized mortality rate gradually decreased after a brief increase (P<0.05). Lung cancer, colorectal cancer, gastric cancer, and liver cancer were the main types of malignant tumors ranking in the top five in terms of incidence and mortality by gender. Multivariate Cox regression results indicate that male, age ≥60 years old, college education level or above, smoking history, drinking history, having been hit by major stressful events, central obesity, hypertension, and coronary heart disease are risk factors for the onset or death of malignant tumors (HR>1). Married, with family size ≥4, frequent consumption of fresh fruit, frequent consumption of fresh vegetables, frequent consumption of grains and tubers are protective factors for the onset or death of malignant tumors (HR<1).ConclusionThe incidence of malignant tumors among middle-aged and elderly people in Chengguan district of Lanzhou from 2011 to 2021 showed an increasing trend, while the overall mortality was decreasing. Our study indicates early cancer screening in elderly populations, maintaining a healthy lifestyle and strengthening the management of chronic diseases are crucial for the prevention and treatment of malignant tumors.关键词：malignant tumors;incidence;mortality;risk factors;middle-aged and elderly people;cohort study- 47
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发布时间：2024-08-12 - “在骨质疏松治疗领域，重庆医科大学附属第一医院的专家通过回顾性分析471例患者资料，揭示了唑来膦酸治疗后急性相反应的独立危险因素，并发现其对治疗依从性和持久性影响不大。”
摘要：ObjectiveTo investigate risk factors for acute phase reaction (APR) induced by zoledronic acid (ZA) treatment in osteoporosis and its impact on treatment adherence and persistence.MethodsA retrospective analysis was conducted using clinical data from 471 osteoporosis patients with a median age of 67 years, who received 5 mg ZA in the First Affiliated Hospital of Chongqing Medical University between January 2013 and January 2022. Patients were divided into two groups based on the occurrence of APR: APR group (n=79) and non-APR group (n=392). Univariate and multivariate logistic regression analyses were used to identify factors inducing APR. The treatment persistence of ZA was analyzed using Kaplan-Meier curves. In addition, treatment adherence was analyzed.ResultsThe incidence of APR was 33.2% after the first ZA injection, and it gradually decreased with an increase in the number of infusions. The multivariate logistic regression analysis showed that the first infusion, no history of oral bisphosphonate use, and high blood phosphorus levels were independent risk factors for APR. Three-year treatment adherence and persistence in APR group were 53% and 44%, respectively, while in non-APR group, they were 52% and 42%, respectively. Kaplan-Meier analysis showed no statistically significant difference in the persistence of ZA between two groups (P>0.05).ConclusionsAPR is prone to occur during ZA treatment for osteoporosis, but it does not affect the adherence and persistence of ZA treatment. First use of ZA, no history of oral bisphosphonates, and high blood phosphorus levels are independent risk factors for APR.关键词：zoledronic acid;acute phase reaction;osteoporosis;risk factors;adherence- 39
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发布时间：2024-08-12 - “在糖尿病防治领域，中国1999-2019年疾病负担分析显示，糖尿病发病率和患病率呈上升趋势，男性疾病负担高于女性，且差距扩大。研究为糖尿病防治和资源分配提供参考，以减轻疾病负担。”
摘要：ObjectiveTo analyze the prevalence, disease burden, and trends of diabetes in China from 1999 to 2019, providing reference for diabetes prevention and control as well as health resource allocation to alleviate the disease burden.MethodsData on the disease burden of diabetes in China from 1999 to 2019 were collected from the Global Burden of Disease (GBD) 2019 database. Indicators such as incidence rate, prevalence rate, mortality rate, disability-adjusted life years (DALYs), years of life lost due to premature mortality (YLLs) and years lived with disability (YLDs) were used to analyze the diabetes burden in China by gender, age and year. The estimated annual percentage change (EAPC) was used to measure the temporal trend of standardized rates over a specified period, and the rank sum test was used to assess the gender differences in disease burden.ResultsIn 2019, 3.78×106 cases of diabetes and 1.73×105 deaths were reported in China. Compared with 1999, the standardized rates of incidence and prevalence increased by 5.80% and 17.12%, respectively, while the standardized mortality rate decreased by 2.82%. Compared with 1999, the standardized rates of DALYs and YLDs increased by 2.86% and 16.06%, respectively, while the standardized YLL rate decreased by 14.39%. The EAPC for diabetes incidence, prevalence, YLDs, and DALYs in the 20-40 age group all exceed 0, indicating an annually upward trend in disease burden of diabetes. Additionally, the disease burden in males was significantly higher than that in females(P<0.05), and the gap is continuously widening.ConclusionsThe disease burden of diabetes in China is severe, particularly in term of life loss due to disability. The population with diabetes onset is becoming increasingly younger. Males constitute a high-risk population for diabetes-related morbidity and mortality. Diabetes prevention and control efforts should continue to enhance behavioral interventions, appropriately tilt health resource allocation towards high-risk population, and focus on improving the rehabilitation capabilities and promoting rehabilitation technology at grass-roots unit.关键词：diabetes mellitus;burden of disease;morbidity;prevalence;disability-adjusted life year- 42
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发布时间：2024-08-12 - “在医学影像学领域，解放军总医院第一医学中心风湿免疫科报道了一例罕见的IgG4相关性肝脏炎性假瘤合并肝组织感染病例。该64岁女性患者表现为多发肺、肝结节，经病理和病原学宏基因测序确诊。治疗后结节基本消失。目前未见类似病例报道，为IgG4-RD肝脏受累合并感染的诊断和治疗提供了新见解。”
摘要：ObjectiveTo report the clinical and imaging characteristics of a case with the clinical manifestation of multiple lung and liver nodules, diagnosed as IgG4-related hepatic inflammatory pseudotumor (HIPT) complicated by hepatic tissue-infection, and review the literature in order to facilitate clinical diagnosis and differential identification of IgG4-related HIPT.MethodsA retrospective analysis was conducted on the medical records of a patient with IgG4-related disease (IgG4-RD) characterized by multiple lung and liver nodules. A literature review was performed by searching Chinese and English databases to summarize the clinical and imaging characteristics of IgG4-related HIPT and hepatic tissue infection.ResultsThe case involved a 64-year-old female admitted to the Rheumatology and Immunology Department of the First Medical Center of Chinese PLA General Hospital due to "poor appetite and fatigue for over a year, and dry cough for four months". She presented with multiple nodules in the lungs and liver, without involvement of the eyelids, salivary glands, submandibular glands, or pancreas. Laboratory test results revealed elevated serum IgG4 levels at 14.1 g/L and C-reactive protein (CRP) at 82.1 mg/L. Pulmonary CT scans indicated multiple solid nodules in both lungs with clear boundaries. Abdominal contrast-enhanced MRI revealed a nodule in liver segment S7 with a pseudocapsule around it, clear boundaries, and uniform enhancement; another nodule in liver segment S5 with blurred boundaries and ring enhancement. The final diagnosis of the liver nodules was confirmed by pathological and metagenomic sequencing to be an IgG4-related HIPT in segment S7 and hepatic tissue infection in segment S5. After a full course of anti-infection and treatment with methylprednisolone and leflunomide, follow-up imaging showed near-complete resolution of the lung and liver nodules. Literatures were searched in China National Knowledge Infrastructure (CNKI), Wanfang, and PubMed databases (up to September 2023), and no case of IgG4-RD complicated with both liver involvement and infection was found. A total of 26 cases of IgG4-RD involving the liver have been reported so far, predominantly in males (92.3%), with an average age of 51 years. Most patients presented with abnormal liver function as the initial symptom, with normal blood inflammatory markers. Imaging typically shows a single nodule in 88.5% of cases, with clear boundaries and uniform enhancement, as well as ring enhancement. Concurrent involvement of the pancreas and biliary tract is common. Pathology is the gold standard for confirming the disease.ConclusionsThis case reports coexistence of IgG4-related HIPT and infection within multiple hepatic nodules. In the diagnosis and treatment of patients with IgG4-RD presenting with multiple hepatic nodules, if the imaging characteristics of the nodules are inconsistent, it is necessary to consider the possibility of the underlying disease coexisting with other conditions, which can be easily misdiagnosed. Actively obtaining pathological tissue is crucial for aiding in the definitive diagnosis.关键词：IgG4-related disease;hepatic inflammatory pseudotumor;infection- 37
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发布时间：2024-08-12 - “最新研究显示，标准化丰富环境显著提升卒中后痴呆患者认知功能，改善日常生活能力，缓解抑郁情绪，提高生活质量，可能与血清BDNF水平升高有关。”摘要：ObjectiveTo explore the effects of standardized environmental enrichment (EE) on cognitive function and serum brain-derived neurotrophic factor (BDNF) levels in patients with post-stroke dementia.MethodsA prospective study was conducted, including 80 patients with post-stroke dementia admitted to Department of Traditional Chinese Medicine Rehabilitation, 910th Hospital of the Joint Logistics Support Force of Chinese PLA from January 2021 to May 2023. Patients were randomly divided into control group, cognitive training (COG) group, aerobic exercise training (AE) group and environmental enrichment (EE) group, with 20 cases in each group. All patients received routine treatment, with COG group receiving additional cognitive function training (30 minutes each time), AE group receiving additional aerobic exercise training (30 minutes each time), and EE group receiving both aerobic exercise and cognitive function training (15 minutes of aerobic exercise training and 15 minutes of cognitive training each time). The training was conducted once a day, 5 days a week, for a total of 8 weeks. The patients' mini-mental state scale (MMSE), modified Barthel index (MBI), Hamilton depression scale (HAMD), stroke-specific quality of life (SS-QOL) score and serum levels of BDNF were assessed before treatment, at 4 weeks and 8 weeks of treatment, respectively.ResultsBefore treatment, there were no significant differences in general information, MMSE, MBI, HAMD, SS-QOL scores, and serum levels of BDNF among the four groups (P>0.05). After 4 and 8 weeks of treatment, the above indicators of the four groups were improved compared with those before treatment, with all differences being statistically significant (P<0.05). Inter-group comparison showed that after 4 and 8 weeks of treatment, MMSE, MBI, SS-QOL scores, and serum BDNF levels in COG, AE and EE groups were significantly higher than those in control group, and HAMD scores were significantly lower than those in control group (P<0.05). In addition, MMSE, MBI, SS-QOL scores and BDNF levels of group EE were better than those of other 3 groups, while HAMD scores were lower than those of other 3 groups, with all differences being statistically significant (P<0.05). There was no significant difference in above outcome indicators between COG group and AE group after 4 and 8 weeks of treatment (P>0.05).ConclusionStandardized enrichment environment can significantly enhance cognitive function, daily living abilities of post-stroke dementia patients, alleviate depression symptoms, and improve the quality of life, which may be related to the increase in serum BDNF levels.关键词：environmental enrichment;post-stroke dementia;cognitive function;BDNF
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发布时间：2024-08-12
Clinical Research
- “最新研究发现，miR-34a/SIRT1在重症监护病房获得性衰弱中扮演关键角色，为治疗提供新思路。”
摘要：ObjectiveTo investigate the role and underlying mechanisms of miR-34a/SIRT1 in intensive care unit acquired weakness (ICU-AW).Methods(1) C2C12 mouse skeletal muscle cells were induced to differentiate into myotubes, and were divided into two groups: model group [ICU-AW group, treated with lipopolysaccharides (LPS) for 12 hours] and normal control group (treated with the same amount of sterile water for 12 hours). Western blotting was used to detect the protein expression level of Muscle ring finger 1 (MuRF-1), atrophy gene 1 (Atrogin-1) and Sirtuin-1 (SIRT1). RT-qPCR was used to assess the mRNA expression level of microRNA-34a (miR-34a), MuRF-1, Atrogin-1 and SIRT1, and light microscope was used to observe the growth and differentiation of C2C12 skeletal muscle cells in each group. (2) ICU-AW cells were further subdivided into control group (treated with siRNA transfection agent intervention), Scra siRNA group (treated with transfection agent and non-specific siRNA), miR-34a siRNA group (treated with transfection agent and specific siRNA intervention), vehicle group (treated with agonist solvent dimethyl sulfoxide) and SRT1720 group (treated with SIRT1 agonist SRT1720). Western blotting was used to detect the protein expression level of SIRT1, Atrogin-1 and MuRF-1 in each group. RT-qPCR was used to detect the miR-34a and the mRNA expression level of SIRT1, Atrogin-1 and MuRF-1 in each group. (3) In addition, another group of ICU-AW cells were divided into control group (treated with siRNA transfection), miR-34a siRNA group (treated with transfection agent and specific siRNA intervention), miR-34a siRNA+vehicle group (treated with transfection agent, specific siRNA and Dimethyl sulfoxide intervention) and miR-34a siRNA+EX-527 group (treated with transfection agent, specific siRNA and SIRT1 inhibitor EX-527). Western blotting was used to detect the protein expression level of Atrogin-1 and MuRF-1. RT-qPCR was used to assess the mRNA expression level of Atrogin-1 and MuRF-1.ResultsMyotube differentiation was observed on the 4th day. Compared with control group, myotube atrophy was obvious in ICU-AW group. RT-qPCR and Western blotting results revealed that, compared with normal control group, in ICU-AW group, the mRNA and protein expression levels of Atrogin-1 and MuRF-1 significantly increased (P<0.05), and the expression level of miR-34a significantly increased (P<0.05), while the mRNA and protein expression levels of SIRT1 significantly decreased (P<0.05). RT-qPCR results showed that, compared with control group (treated with siRNA transfection agent intervention) and Scra siRNA group, the expression of miR-34a and mRNA expression of Atrogin-1 and MuRF-1 in miR-34a siRNA group significantly decreased (P<0.05), while the mRNA expression of SIRT1 significantly increased (P<0.05), meanwhile the protein expression of Atrogin-1 and MuRF-1 decreased significantly (P<0.01), and the protein expression of SIRT1 significantly increased (P<0.05). RT-qPCR results also showed that, compared with vehicle group, the mRNA expression of Atrogin-1 and MuRF-1 in SRT1720 group decreased significantly (P<0.05), while SIRT1 increased significantly (P<0.05). Western blotting results demonstrated that, compared with control group and Scra siRNA group, the protein expression of Atrogin-1 and MuRF-1 in miR-34a siRNA group decreased significantly (P<0.05), while SIRT1 increased significantly (P<0.05). RT-qPCR and Western blotting results indicated that, compared with miR-34a siRNA+vehicle group, the mRNA and protein expression of Atrogin-1 and MuRF-1 in miR-34a siRNA+EX-527 group increased significantly (P<0.05).ConclusionOveractivation of miR-34a in ICU-AW contributes to skeletal muscle atrophy by inhibiting the expression of SIRT1, which may play an important role in the pathogenesis of ICU-AW.关键词：intensive care unit acquired weakness;muscular atrophy;miR-34a;Sirtuin-1;atrophy gene 1;muscle ring finger 1- 44
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发布时间：2024-08-12 - “最新研究发现，柴黄益肾颗粒能有效减缓单侧输尿管梗阻小鼠肾纤维化，为治疗肾纤维化提供新策略。”
摘要：ObjectiveTo investigate the effects of Chaihuang Yishen Granules on renal fibrosis in unilateral ureteral obstruction (UUO) mice and its underlying mechanisms.MethodsTwenty-four 8-week-old male C57BL/6 mice were randomly divided into control group, model group, and low and high dose groups of Chaihuang Yishen Granules (6 in each group). In control group, only right kidney ureter was exposed and dissected. In model group, the UUO animal model was established by UUO. In low and high dose groups, mice were administered intragastrically at doses of 3.8 and 7.6 g/kg of Chaihuang Yishen Granules respectively,following the model group's method to establish the UUO model. After 7 days, the mice were euthanized and renal samples were collected. HE and Masson staining were used to observe pathological changes and fibrosis degree of the kidneys in each group, Sirius red staining was used to observe collagen deposition. The expression levels of α-smooth muscle actin (α-SMA), fibronectin (FN), type Ⅰ collagen (Col-Ⅰ), glycogen synthase kinase 3β (GSK-3β), and β-catenin related proteins were detected using Western blotting. Changes in A33 and GSK-3β, β-catenin mRNA levels were measured by RT-PCR. Additionally, a normal transformed C3H mouse kidney-1 (TCMK1) was used as control (normal group); an in vitro fibrosis model was established using TCMK1 stimulated with Transforming Growth Factor‑β (TGF‑β); and an in vitro drug model was established using TCMK1 treated with serum containing Chaihuang Yishen Granules. A33 was overexpressed in TCMK1 cells using a transfection with an A33 overexpression plasmid, and changes in fibrosis-related indicators and the expression of A33 and GSK-3β, β-catenin mRNA were observed.ResultsRT-PCR results showed that, compared with control group, A33 level was significantly increased in model group, while it was significantly reduced in both low and high dose groups of Chaihuang Yishen Granules (P<0.05). Western blotting showed that the expression levels of fibrosis-related factors such as α-SMA, FN, Col-Ⅰ in model group were significantly higher than those in control group (P<0.05); while compared with model group, the expression levels of α-SMA, FN, Col-Ⅰ in low and high dose groups of Chaihuang Yishen Granules were significantly lower (P<0.05). HE, Masson, immunohistochemical staining results showed that model group had severe kidney structural damage, significant increase in collagen deposition, and significantly higher expression levels of GSK-3β and β-catenin proteins compared with those in control group (P<0.01). In contrast, low and high dose groups of Chaihuang Yishen Granules had good kidney structure, significant improvement in kidney damage and fibrosis, and significantly lower expression levels of GSK-3β and β-catenin proteins compared with those in model group (P<0.05). In vitro experiment results confirmed that, compared with normal group, A33 overexpression promoted the upregulation of fibrosis-related factors in TCMK1 cells, significantly increase the expression of downstream target genes GSK-3β and β-catenin mRNA in the Wnt/β-catenin signaling pathway (P<0.05), and A33 overexpression reversed the cellular fibrosis changes downregulated by the serum containing Chaihuang Yishen Granules (P<0.01).ConclusionChaihuang Yishen Granules significantly improve renal fibrosis in UUO mice by downregulating the A33/Wnt/β-catenin signaling pathway, suggesting that A33 may be a potential therapeutic target for renal fibrosis.关键词：Chaihuang Yishen Granule;renal fibrosis;Wnt/β-catenin signaling pathway- 45
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发布时间：2024-08-12 -
Effects of inhibiting
KDM2A gene on the proliferation, invasion, and migration of liver cancer cells AI导读“最新研究发现，抑制KDM2A基因表达可显著降低肝癌细胞的增殖、侵袭和迁移能力，miR-29a-3p可能是其调控的关键因子，为肝癌治疗提供新思路。”
摘要：ObjectiveTo investigate the effects of inhibiting KDM2A gene on the proliferation, invasion and migration of liver cancer cells and its possible regulatory mechanism.MethodsForty pairs of HCC tissues and their adjacent normal counterparts were collected from 2014 to 2017 in Tongji Hospital, Tongji Medical College Affiliated to Huazhong University of Science and Technology. Human liver cancer cell lines HepG2, Huh7, HCCLM3, MHCC-97H and normal liver cells LO2 were cultured in vitro. The mRNA and protein expression levels of KDM2A in HCC tissues and cells were detected by qRT-PCR and Western blotting. Huh7 cells were taken and set up as follows: (1) si-NC group (transfected with si-NC) and si-KDM2A group (transfected with si-KDM2A); (2) mimic-NC group (transfected with mimic-NC), miRNA-29a-3p mimic group (transfected with miRNA-29a-3p mimic), inhibitor-NC group (transfected with inhibitor-NC) and miRNA-29a-3p inhibitor group (transfected with miRNA-29a-3p inhibitor). The mRNA and protein expression levels of KDM2A were detected by qRT-PCR and Western blotting. The invasion and migration of cells were detected by Transwell, the proliferation of cells was detected by CCK-8 methods. The online databases TargetScan, miRDIP, miRWalk, Starbase and miRDB were used to predict the binding sites of KDM2A and miR-29a-3p. The KDM2A 3'-UTR (WT) or KDM2A 3'-UTR (MUT) report plasmid was co-transfected with NC-miRNA or miR-29a-3p mimics respectively for 48 h in 293T cells, and the luciferase activity was detected by the luciferase reporter gene detection system.ResultsCompared with adjacent normal counterparts, the relative mRNA and protein expression levels of KDM2A in HCC tissues increased significantly (P<0.05). Compared with LO2, the relative mRNA and protein expression levels of KDM2A in HepG2, Huh7, HCCLM3 and MHCC-97H increased significantly (P<0.05). Compared with si-NC group, the proliferation, invasion and migration of Huh7 cells in si-KDM2A group decreased significantly (P<0.05 or P<0.01). The analysis results of TargetScan, miRDIP, miRWalk, Starbase and miRDB showed that there were binding sites between KDM2A and miR-29a-3p. The results of the dual luciferase reporter assay showed that miR-29a-3p mimic significantly reduced KDM2A-MUT luciferase activity (P<0.01). After overexpression of miRNA-29a-3p, the relative mRNA and protein expression levels of KDM2A were decreased (P<0.01), the proliferation, invasion and migration abilities were decreased (P<0.05) in Huh7 cells. After inhibiting the expression of miRNA-29a-3p, the relative mRNA and protein expression levels of KDM2A were increased (P<0.05), the proliferation, invasion and migration abilities were enhanced (P<0.05) in Huh7 cells.ConclusionInhibiting the expression of KDM2A can reduce the proliferation and migration ability of Huh7 cells. miR-29a-3p may be the upstream regulator of KDM2A and participate in the occurrence and development of hepatocellular carcinoma.关键词：KDM2A;miR-29a-3p;hepatocellular carcinoma;proliferation;invasion and migration- 37
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发布时间：2024-08-12 - “最新研究发现，X染色体失活特异转录本XIST通过调控miR-132-3p影响椎间盘退变，为治疗提供新策略。”
摘要：ObjectiveTo investigate the influences and mechanism of X chromosome inactivation specific transcript (XIST) on the proliferation and extracellular matrix synthesis of nucleus pulposus cells in rats with intervertebral disc degeneration (IDD).MethodsSD rat intervertebral disc nucleus pulposus cells were isolated and cultured in vitro, and then randomly divided cells into control group, model group, XIST knockdown group, empty plasmid group, and XIST knockdown+miR-132-3p knockdown group. Except for control group, the cells in other groups were induced by interleukin (IL)-1β to establish IDD models. After nucleus pulposus cells were grouped and treated, the expressions of XIST and miR-132-3p in the nucleus pulposus cells of rats were detected by qRT-PCR; the proliferation of intervertebral disc nucleus pulposus cells was detected by MTS method and EdU staining; ELISA was used to measure the levels of inflammatory factors IL-6 and IL-18 in the intervertebral disc nucleus pulposus cells; Immunoblotting was used to detect the expression of extracellular matrix labeled proteins Collagen Ⅱ and Aggrecan in rat nucleus pulposus cells. The targeted regulation of miR-132-3p by XIST in rat nucleus pulposus cells was detected by dual-luciferase reporter gene assay. IDD rat models were established by intramuscular injection of IL-1β and divided into sham operation group, model group, XIST knockdown group, no-load plasmid group, XIST knockdown+miR-132-3p knockdown group, with 12 rats in each group. After treatment in each group, the expressions of XIST and miR-132-3p in intervertebral disc tissues were detected by qRT-PCR; TUNEL staining was used to detect apoptosis of nucleus pulposus cells in intervertebral disc tissue of rats; the morphology of intervertebral disc cartilage was observed by saffron O staining; serum levels of inflammatory factors IL-6 and IL-18 were determined by ELISA; the expressions of Collagen Ⅱ and Aggrecan in intervertebral disc tissues were detected by Western blotting.ResultsCompared with control group (cells)/sham operation group (rats), XIST expression in intervertebral disc tissue and nucleus pulposus cells, apoptosis rate of intervertebral disc nucleus pulposus cells, levels of inflammatory factors IL-6 and IL-18 in intervertebral disc nucleus pulposus cells culture medium and serum were increased in model group (P<0.05), the activity and proliferation rate of nucleus pulposus cells and the expressions of miR-132-3p, Collagen Ⅱ and Aggrecan protein in nucleus pulposus cells and intervertebral disc tissues decreased (P<0.05); compared with model group, the apoptosis rate of nucleus pulposus cells, the levels of inflammatory factors IL-6 and IL-18 in intervertebral disc nucleus pulposus cells culture medium and serum, and the expression of XIST in nucleus pulposus cells and intervertebral disc tissues of rats in XIST knockdown group decreased (P<0.05), the activity and proliferation rate of nucleus pulposus cells and the expressions of miR-132-3p, Collagen Ⅱ and Aggrecan protein in nucleus pulposus cells and intervertebral disc tissues increased (P<0.05). Down-regulation of miR-132-3p attenuates the ameliorative effect of knockdown XIST on IL-1β-induced intervertebral disc injury and cartilage matrix loss. XIST was able to target and down-regulate the expression of miR-132-3p in rat nucleus pulposus cells.ConclusionKnockdown of XIST can inhibit inflammation by up-regulating miR-132-3p, thereby inhibiting the apoptosis of IDD nucleus pulposus cells and promoting their proliferation and extracellular matrix synthesis.关键词：XIST;miR-132-3p;intervertebral disc degeneration;nucleus pulposus cells;proliferation;extracellular matrix synthesis- 22
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发布时间：2024-08-12 - “最新研究发现，WDR82蛋白在神经胶质瘤中高表达，可能通过Akt/mTOR信号通路促进肿瘤生长，为治疗提供新思路。”
摘要：ObjectiveTo investigate the role and underlying mechanisms of WD repeat domain 82 (WDR82) protein in the pathogenesis and progression of glioma.MethodsWe analyzed the expression level of WDR82 in glioma tissues using GEPIA and UALCAN databases and further assessed WDR82 protein expression in glioma and adjacent normal tissues through immunohistochemical staining. The correlation between WDR82 expression and the prognosis of glioma patient was evaluated using Kaplan-Meier plotter. Experiments were conducted on A172 and U251 cell lines, which were categorized into four groups: control group (transfected with 3 μg pcDNA3), shR-control group (transfected with 3 μg pSilencer 2.1-U6), pWDR82 group (transfected with 3 μg pWDR82), and shR-WDR82 group (transfected with 3 μg shR-WDR82). Post-transfection, we confirmed transfection efficiency at 48 hours using qRT-PCR and measured cell viability at the same time point with CCK-8 assay. Clone formation assay was employed to assess cell proliferation capacity after 14 days of transfection, while flow cytometry was utilized to analyze cell apoptosis after 48 hours of transfection. Additionally, Western blotting was conducted to determine the expression levels of proteins related to proliferation and Akt/mTOR signaling pathway after 48 hours of transfection. Finally, the effect of WDR82 on tumor growth in NOD-SCID mice was investigated using tumor carrying experiment in vivo.ResultsAnalysis of WDR82 expression in glioma tissues using GEPIA and UALCAN databases, along with immunohistochemical staining, revealed significantly higher expression levels compared to normal and paracancerous tissues (P<0.05). Additionally, WDR82 expression was not associated with gender or age of patients (P>0.05). Kaplan-Meier plotter analysis indicated that elevated WDR82 expression correlated with a poor prognosis in glioma patients (log-rank P=0.029). Overexpression of WDR82 notably enhanced the proliferation and inhibited the apoptosis of A172 and U251 cells, and also significantly upregulated the expression of MKI67, BCL2, CCND1, p-Akt and p-mTOR in A172 and U251 cells (P<0.05). Conversely, WDR82 knockdown had the opposite effects, inhibiting cell proliferation, increasing apoptosis and downregulating the expression of MKI67, BCL2, CCND1, p-Akt and p-mTOR (P<0.05). WDR82 knockdown in U251 cells significantly inhibited tumor growth in NOD-SCID mice (P<0.05).ConclusionHigh expression of WDR82 promotes the proliferation of glioma cells and the growth of tumors in vivo by regulating the AKT/mTOR signaling pathway.关键词：WDR82;glioma;proliferation;apoptosis;Akt/mTOR signaling pathway;mouse, NOD-SCID- 26
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发布时间：2024-08-12
Basic Research
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Application of smart responsive materials in the precise repair of osteonecrosis of the femoral head AI导读
“在骨科领域，智能响应性材料的研究取得新进展，为股骨头坏死精准修复提供新思路。”摘要：Osteonecrosis of the femoral head (ONFH) is a common orthopedic disease, and hip preservation surgery has high clinical value in the early stages of ONFH, especially for young and middle-aged patients. However, the repair of ONFH is heterogeneous, leading to inter-individual variations in the efficacy of hip preservation. Currently, the existing tissue-engineered scaffolds in the field of hip preservation are uncontrollable after implantation, making it difficult to achieve precise repair. Smart responsive materials have good biocompatibility and self-feedback capability. By combining them with therapeutic drugs to construct stimulus-responsive drug delivery systems, new possibilities are provided for the precise repair of ONFH. This paper reviews the research progress of smart responsive materials at home and abroad. Based on the response principles of various materials and the repair characteristics of ONFH, the application prospects of various smart responsive materials such as reactive oxygen species-responsive, fluid shear stress-responsive, and light/magnetic-responsive materials are discussed and prospected in the field of precise repair for ONFH, providing new ideas for the precise treatment of ONFH.关键词：smart responsive materials;femoral head osteonecrosis;heterogeneity repair;precise repair;application prospects- 38
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发布时间：2024-08-12 -
Research progress of tumor autoantibodies and CT artificial intelligence in early diagnosis of NSCLC AI导读
“在非小细胞肺癌早期诊断领域，CT人工智能与7种自身抗体试剂盒的研究进展，为提高诊断准确性和降低漏诊率提供了新思路。”摘要：Non-small cell lung cancer ( NSCLC ) is one of the most prevalent and deadly malignant tumors in the world. In recent years, artificial intelligence (AI) in computed tomography (CT) has harnessed the power of big data to automatically extract and learn imaging features, thereby assisting radiologists in reducing the workload and missed diagnosis rate of pulmonary nodules. An ELISA kit for detecting seven lung cancer autoantibodies (p53, SOX2, PGP9.5, CAGE, MAGE-A1, GAGE7, and GBU4-5) has been clinically implemented in China, showing high specificity in the early screening of NSCLC. Additionally, other liquid biopsy techniques such as circulating tumor DNA (ctDNA) methylation markers are also continually being explored. However, existing methods for the early diagnosis of lung cancer all have their limitations, and optimizing their combination or establishing diagnostic models has become a trend. This review summarizes the research progress and value of the seven lung cancer autoantibodies and CT AI in the early diagnosis of NSCLC, with the aim of providing a reference for their combined use in the early diagnosis of lung cancer in Chinese population.关键词：non-small-cell lung carcinoma;autoantibodies;artificial intelligence;pulmonary nodules;early diagnosis- 35
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发布时间：2024-08-12
Review
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